LOCUS       sequence-75062-e        2923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30010
FEATURES             Location/Qualifiers
     source          1..2923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(160..951)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1171..1275)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1343..1361
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2153..2170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2324..2912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2404..2423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
       61 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      121 atcaatctaa agtatatatg agtaaacttg gtctgacagt tatttgccga ctaccttggt
      181 gatctcgcct tttacgtaat ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
      241 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
      301 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
      361 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
      421 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
      481 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
      541 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
      601 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
      661 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
      721 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
      781 ccttacggtc accgtaacca gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac
      841 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
      901 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa
      961 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     1021 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     1081 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     1141 caaggttctg gaccagttgc gtgagcgcat actcttcctt tttcaatatt attgaagcat
     1201 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1261 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     1321 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc cactgaagag
     1381 ccactgtggt ctcaccatgt ctgcaattaa gcctgacatg aagatcaagt tgaggatgga
     1441 gggaaacgtg aacggacacc acttcgtgat cgacggtgac ggaactggta agcctttcga
     1501 gggaaagcag tcaatggact tggaggtgaa ggagggagga cctttgccat tcgctttcga
     1561 catcttgact actgctttcc actacggtaa cagggttttc gctaagtacc ctgataacat
     1621 tcaagattac ttcaagcagt cattcccaaa gggttactct tgggagagat cattgacttt
     1681 cgaggacgga ggtatctgca tcgcaaggaa cgacatcaca atggagggag acacatttta
     1741 caacaaggtg aggttctacg gaactaactt ccctgcaaac ggacctgtga tgcagaaaaa
     1801 aacattgaaa tgggagcctt ctacagagaa gatgtacgtg agggacggag tgttgactgg
     1861 tgacatccac atggcattgt tgcttgaggg taacgctcac tacaggtgcg acttcaggac
     1921 tacatacaag gctaaggaga agggagtgaa gttgcctgga taccacttcg ttgaccactg
     1981 catcgagatt ttgtctcacg acaaggacta caataaggtt aagttgtacg agcatgcagt
     2041 ggcacactct ggattgcctg acaacgctag gagaatgtga gaccacgagt gattaatgaa
     2101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2761 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc ttt
//
