LOCUS       sequence-75104-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Akt1 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLPS-hAkt1-gfp
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    221..254
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(349..368)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgctcgcga ccggtagctg tggaaaaaaa agggacagga taagtatgat attgtcaagg
       61 aaaccctgaa ttcgaagctt gagctcgaga tccacgtcag gtggcacttt tcggggaaat
      121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      181 agacaataac cctgataaat gcttcaataa tattggatct ataacttcgt ataatgtatg
      241 ctatacgaag ttatggatct ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      301 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      361 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      421 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      481 atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat
      541 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      601 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      661 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      721 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      781 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgaca
//
