LOCUS       sequence-75106-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Wang NS, Unkila MT, Reineks EZ, Distelhorst CW
  TITLE     Transient expression of wild-type or mitochondrially targeted Bcl-2 
            induces apoptosis, whereas transient expression of endoplasmic 
            reticulum-targeted Bcl-2 is protective against Bax-induced cell 
            death.
  JOURNAL   J Biol Chem. 2001 Nov 23. 276(47):44117-28.
  PUBMED    11546793
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Nov 23. 276(47):44117-28."
COMMENT     Sequence Label: Flag-Bcl2
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..66
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gattacgcca agctcgaaat taaccctcac
       61 taaagggaac aaaagctgga gctccaccgc ggtggcggcc gccaccatgg attacaagga
      121 tgacgacgat aagagcccgg gcggatctat ggcgcacgct gggagaacgg ggtacgataa
      181 ccgggagata gtgatgaagt acatccatta taagctgtcg cagaggggct acgagtggga
      241 tgcgggagat gtgggcgccg cgcccccggg ggccgccccc gcaccgggca tcttctcctc
      301 ccagcccggg cacacgcccc atccagccgc atcccgcgac ccggtcgcca ggacctcgcc
      361 gctgcagacc ccggctgccc ccggcgccgc cgcggggcct gcgctcagcc cggtgccacc
      421 tgtggtccac ctgaccctcc gccaggccgg cgacgacttc tcccgccgct accgccgcga
      481 cttcgccgag atgtccagcc agctgcacct gacgcccttc accgcgcggg gacgctttgc
      541 cacggtggtg gaggagctct tcagggacgg ggtgaactgg gggaggattg tggccttctt
      601 tgagttcggt ggggtcatgt gtgtggagag cgtcaaccgg gagatgtcgc ccctggtgga
      661 caacatcgcc ctgtggatga ctgagtacct gaaccggcac ctgcacacct ggatccagga
      721 taacggaggc tgggatgcct ttgtggaact
//
