LOCUS       sequence-75108-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of human Sox2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-hSox2-ires-gfp
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    34..586
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    36..603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(196..215)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     425..442
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gctcctcgcc cttgctcacc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa
       61 accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta
      121 aagcatgtgt accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc
      181 ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa
      241 ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt
      301 tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca
      361 gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga
      421 ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg
      481 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
      541 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg ggggggggag
      601 ggagag
//
