LOCUS       sequence-75114-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of mouse Olig2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-mOlig2-ires-gfp
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(19..27,28..30,31..606)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(192..211)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     421..438
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    741..774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctcgcccttg ctcaccatgg ttgtggccat attatcatcg tgtttttcaa aggaaaacca
       61 cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc
      121 atgtgtaccg aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca
      181 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
      241 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
      301 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
      361 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
      421 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
      481 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
      541 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggga
      601 gaggggcgga tccacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      661 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      721 gcttcaataa tattggatct ataacttcgt ataatgtatg ctatacgaag ttatggatct
      781 ttca
//
