LOCUS       sequence-75133-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant lacking cysteine residues within 
            intracellular loops and with an N-terminal 3xFLAG-YFP tag in 
            mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 intracellular-cystein...
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtccatgtgt acgtggccag agatcatcaa accaacattt tttgtgaaat cgtgaacaag
       61 atgaagtaag gccggccgtg agtttggcgc tcctgtcata acaagggact gtggcctaaa
      121 gtttttcaca tggtcttcca cgccagaaag cctaatacta tgttgcaggg cattgaggta
      181 agtcagggct tgtgtagagg atccccaatt cacatcgggt tttttgtagg taacataaat
      241 atacagccca aggactatca cgtaggttag caaagcagcc caccagttaa tgacgaacat
      301 tactatgcaa caaagaattg ctccaagaag tgagatccac atattgtagt atttaaatgc
      361 cggtcgccat cctgggcttt ttgccagtga agcatggaat actgaaaaat tgatgagggc
      421 atagctggca aggaagaagt ttgagataat tggtgcaata acattcagtt cggcaattaa
      481 gatgaatcca agtgcaatta agaatgttaa gatgtagcca cgtaatggct cattattttt
      541 cccataacct ttagcaaaca tctggaaagc ggggtagatg ttgtctttac atagggcctg
      601 aaatatcttg ggagcactca ctagggatgc taatgcgcta gaaagagtgg cagagaatat
      661 gcccgcggaa attagtggtg taaatcctga caccatactc attacctgaa agttgttcat
      721 taggccgtag ctacaaggac tggattcaca agatgaaaaa tcaaagttta atttgcaggc
      781 tgcgctagta cagtttgtta gctctgttac gatagtgtca ttaacattac cagtggcatc
      841 gcgtacgaca caa
//
