LOCUS       sequence-75134-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split fluorescence protein to measure caspase-2 
            dimerization in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Bouchier-Hayes L, Oberst A, McStay GP, Connell S, Tait SW, Dillon 
            CP, Flanagan JM, Beere HM, Green DR
  TITLE     Characterization of cytoplasmic caspase-2 activation by induced 
            proximity.
  JOURNAL   Mol Cell. 2009 Sep 24;35(6):830-40. doi: 
            10.1016/j.molcel.2009.07.023.
  PUBMED    19782032
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.07"; journalName: "Mol Cell"; date: 
            "2009-09-24- 24"; volume: "35"; issue: "6"; pages: "830-40"
COMMENT     Sequence Label: pBiFC-HA-hCaspase 2 FL (C303S)-VC155
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..269)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(60..81)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatcccgcgg ccgcttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
       61 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
      121 tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      181 tgctggtagt ggtcggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatcttgaag
      241 ttggccttga tgccgttctt ctgcttgtcg tggttcatga ctttctgttt caggtcgttc
      301 gggattttgc acgccggacg ggtacctcga gagatctctg tgggagggtg tcctgggaac
      361 aggtagaggt ggcggcacag agtgctgcag tattcagaca tctccttgca ccggtggaat
      421 tctgtgccag gagcataacc ttcccgatcc ttgataagtg cgttcacctt aaccagcatg
      481 tcggccacgt gcatatcaca agcccgctca gaaaacactt gagcaagagc ctcgatgtac
      541 caggaacctc gtttggtgtt ccgcatggcg gcagtccctt tgaggcaggc atagccgcat
      601 atcatgtctg agcgcgtggg cagtctcatc ttcggcaact tttctttacc ggcatcactc
      661 tcctcgcacc c
//
