LOCUS       sequence-75136-e        5609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse eIF2C1 cDNA clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pBluescript-mouse eIF2C1
FEATURES             Location/Qualifiers
     source          1..5609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3375..3391)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3376..3392)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3421..3441)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3421..3439)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3460..3476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3460..3476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3473..3495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3484..3500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3508..3514,3515..3532,3533..3538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3553..3574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3691..3708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3862..4450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3942..3961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4621..5412,5413..5481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5244..5263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5482..5586)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatgcttt
      721 cttctatcca gtgaggtaac agcgttctgc cttcaagcga agtacatggt gcgtagagta
      781 tcctggtgaa cctgcacagc tttggccagg gcctgggggt ctcgcccatt gctttgccct
      841 gatatgtggc tcccctctcc actgtcatgt tccttgtcca ccaggtggta tcgtgcccgg
      901 aaagccacca agcgagcata gtaggcaggt gctgggatag agacagaacg tgtgcatcgt
      961 acgtaagtgt ggcacagctg gtatgtcaag atctggagtt catctgctgt gaaacggttg
     1021 tcatcccaaa ggacataata atgggatggt cggctggtac cctggatgcc tgcatggctg
     1081 cacagataga agtcgaactc aaatgggtgg gtgatgttgg tgtccacagt ggtccctgct
     1141 gggatgttac cactcttccc aatccgctca ttcttgtcag cacagaacag gcgggtgtga
     1201 tggcgcttct gcaccacaat atatgtgatc ccaggctggt agtctttttc cagtttgatg
     1261 catgcatctc gaatggcaag cagctcatag tgaagaatct ggggcagctg gccctcgggg
     1321 acgccgtcgc ggtagaagat gatgcgggtg ggcttgaagc gggtggactt gtagaactgg
     1381 ataagcagct cgcgcaccat ataggacaag tcttcaatga tctcctgccg tgggcgctgc
     1441 acacgcacgg tggcacagta tcggctgggg tgtgcgtcca tgctgcctac cactgctgtg
     1501 atagatggtt tcttcccatc cccagctggg ggatgtgtaa catctgctcc caggaaaatc
     1561 actggctgtt gaaaaacagc agagcgctgg tgtgggacta ggatgttgtt aatgccaccg
     1621 agtttgacat tgatcttgag gcagaggttg gacagagtct gcggtgacgt cttgaccgca
     1681 ttcttcacct gcacacactg tgttgccatt cccaagagtg tatctccgac acgtttcact
     1741 tcagcataca ctggcgtctt cccgggcagg atgacgataa tgagctgcag tcctgagtag
     1801 gtattcttga gatgccggaa catgggctcc acgctgtctg ccccctgtgc gtatttgcag
     1861 aagcatggct gaccctggat gggcatccct gcatccttgg aaatcttccg aagctggtct
     1921 gtgaagttct tgagcacctc ttctcgacac tgtttctggg gtgcgaagca ggcgatggcc
     1981 cagactttga tctcaatccc attgtagaac tgtttcccgc gcatgtccca gacaccttgg
     2041 ttgggcgtag caatggcccg gttccggccg ccatactgca agatgggcgc cggcagcaca
     2101 cgtcctgtca cctccgtcat gtcatccttc actttgattc caaattcctg gatgtaggga
     2161 tccaggttgc agctagcatt cttcatcagg cggctgatct cctcctgtct gtctggggcc
     2221 gacctagctg tagcctttat catggttgaa gtctggttgt cagtcagctt cttaatgcac
     2281 cgctgcccag ccacaatgtt acagacctcg aggggcaaat aggtatgctt ctgttcttgc
     2341 ccaacttgta ggcagggcag gtgaggatac ttgagctgaa ggttatattt ctgcttgaaa
     2401 tgctgtgcca cagtgcactc cacagtctgt ccactctcca gctgcaaggg gaacgtctga
     2461 tggctagcag ggcggcgggt aacattacac acgcggtatt tcctcttcat ctgtccacag
     2521 tgggtcactt ccaccttcag gccttttatc tccttggtaa accgaacacg ctgggaatcc
     2581 gtgaggggct tgggttgttc gtctatgttc ctgatgtcca ggacctcaca catgaactca
     2641 atcactggct gtgctttgta gaaggcagtg gctgagacat caatgttgag catcatcttc
     2701 cacatggcag ggcgcacaga ctggtgaaag ccgaaccaga cctcgcgccc accccccagc
     2761 gggtggtagt agccctcagg cggtgagaag aaggagcggc ccacaggggt gtacctcata
     2821 gatgccaggt gcctcatggc cacatccagg gcttgcacag actccagggg cacagggatc
     2881 tggccactga ccaaggcttc atgcagcatg cgccagctca caatggctag ccacttgatg
     2941 gagaccttaa aaattctatc cttcccctcc ccggggattg tcacctcaaa gtcaaccctc
     3001 tcattgccaa tgggcagtgc tgtgaccgtg taaatattct cctttccatc atacacaggc
     3061 ttgcgatccc caaagatctg cggtttgaaa tgctggacca tgtattccac cacctcccgg
     3121 ttgactctgc gaggacactt atccggcttg atgtccacct cgtaatggta aacgtcaatc
     3181 ttaggaatgt ccacctcaaa gtaattggcc agaagcttga ttggtttgcc cacagtgcca
     3241 atgccgggcc ggcggggtgc ctgaaacacc tgctgcaggg gaggcaggta ggcacctgcc
     3301 gctgctcccg agggtcccgc ttccatccca tatacccgtg cggaggtcag gcggcccgga
     3361 gacatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     3421 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5581 tccgcgcaca tttccccgaa aagtgccac
//
