LOCUS       sequence-75139-e        5618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse eIF2C2 cDNA clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pBluescript-mouse eIF2C2
FEATURES             Location/Qualifiers
     source          1..5618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3384..3400)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3385..3401)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3430..3450)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3430..3448)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3469..3485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3469..3485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3482..3504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3493..3509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3517..3523,3524..3541,3542..3547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3562..3583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3700..3717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3871..4459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3951..3970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4630..5421,5422..5490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5253..5272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5491..5595)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgattcggc
      721 cgcggtcctc ggcaacgcca ccatgtactc gggagccggc cccgttcttg cttctcctgc
      781 tccgacaaca tcacccatcc caggatatgc cttcaaacct ccacctcggc cggacttcgg
      841 caccaccggg agaacaatca aactacaggc caatttcttt gaaatggaca tccccaaaat
      901 tgacatctat cactatgaat tggacatcaa acctgagaaa cgccctcgga gagtgaacag
      961 ggaaattgtg gagcacatgg tccagcactt taaaactcag atcttcgggg accggaagcc
     1021 agtgtttgat ggaaggaaga atctgtacac agcaatgccc cttccgatcg gcagggacaa
     1081 ggtggagctg gaggtcacgc tcccgggaga aggcaaagat cgcatcttaa aggtatccat
     1141 caagtgggtg tcgtgcgtga gcctgcaggc gttacacgat gcactttcgg ggcggctgcc
     1201 cagcgtcccc ttcgagacga tccaggccct ggacgttgtc atgaggcact taccatccat
     1261 gaggtacacc cctgttggcc gttccttctt cactgcatct gaaggctgtt ccaaccctct
     1321 gggtgggggc agagaagtgt ggtttggctt ccatcagtcc gtccgacctt ctctttggaa
     1381 aatgatgctg aatattgatg tatcggcaac agcgttttac aaggcacagc cagtgatcga
     1441 gtttgtttgt gaagttttgg attttaaaag tattgaagaa caacaaaaac ctctgacaga
     1501 ttcccaaagg gtaaagttta ccaaagaaat caaaggtcta aaggtggaga taacgcactg
     1561 tggtcagatg aagaggaagt accgtgtctg caatgtgacc cggcggcctg ccagtcacca
     1621 aacgttccca ctgcagcagg agagtgggca gacagtggag tgtacagtgg cccagtactt
     1681 caaggacagg cacaagctgg ttctgcgcta cccccacctc ccgtgtttac aagtcggaca
     1741 ggagcagaaa cacacctacc ttcctttgga ggtctgtaac atagttgctg gacagagatg
     1801 tataaaaaaa ttaacagacg atcagacctc aaccatgatc agagcaactg ccaggtcagc
     1861 acctgatcgc caagaggaga tcagcaaact gatgcgaagt gcaagtttca atacagaccc
     1921 atatgttcgt gaatttggaa tcatggtgaa agatgagatg acagatgtga ctgggcgggt
     1981 tctgcagccg ccctccatcc tctacggggg caggaacaaa gcaattgcca cccctgtcca
     2041 gggtgtgtgg gacatgcgaa acaagcagtt ccacacgggc atcgagatca aggtgtgggc
     2101 catcgcctgc ttcgctcccc agcgccagtg tacagaagtc catctcaagt ccttcacaga
     2161 gcaactgagg aagatctcga gagatgctgg gatgccgatc cagggtcagc cctgcttctg
     2221 taaatacgca cagggtgcag acagtgtgga gcccatgttc cgacacctga agaacacata
     2281 cgctggcctc cagctggtgg tggtcatcct gcctggcaaa actcctgtgt atgcggaagt
     2341 caagcgtgtg ggagacacag tgctggggat ggccacacag tgcgtccaga tgaagaacgt
     2401 gcagaggacc acgccacaga ccctatccaa tctctgctta aagatcaatg tcaaactggg
     2461 aggcgtcaac aacatcctgc tgccacaggg caggcctcca gtgttccagc aacctgtcat
     2521 cttcctggga gccgatgtca cccacccacc agctggggat gggaagaaac cttctattgc
     2581 tgccgttgtg ggcagcatgg acgcacaccc caaccgctac tgtgccaccg tgcgcgtgca
     2641 gcagcaccga caggagatca tccaggacct ggctgccatg gtacgagagt tgctcattca
     2701 gttctacaag tccacccgct tcaagcccac ccgcatcatc ttctaccgcg atggcgtctc
     2761 cgagggccag ttccagcagg ttctccacca tgagctcctg gccatcagag aggcctgcat
     2821 caagctggag aaggactatc agccaggaat cacgttcatc gtggtgcaga agcggcacca
     2881 cacacgcctc ttctgcacag acaaaaatga gcgggttggg aagagtggga acattcccgc
     2941 aggcacaacc gtggacacga agatcaccca ccccactgag tttgacttct acctgtgcag
     3001 tcacgccggc atccagggga caggccgtcc ttcccactac cacgtgcttt gggatgacaa
     3061 tcgtttttct tctgatgagc tgcagattct gacctaccag ctgtgtcaca cctacgtgcg
     3121 ctgcacacgc tctgtgtcaa tacccgcgcc agcttactat gctcacctgg tggccttccg
     3181 agccaggtac cacctggtgg ataaagaaca tgacagcgct gaaggaagcc atacctctgg
     3241 gcagagcaac gggcgagatc accaagcgct ggccaaggcg gtccaggtcc accaggacac
     3301 actgcgcacc atgtactttg cttgacctgt tgtattgttt agcgattgtg taccaagttg
     3361 gcttcactcg agatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccag
     3421 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     3481 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3541 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3601 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3661 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3721 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3781 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3841 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3901 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3961 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4021 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4081 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4141 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4201 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4261 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     4321 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4381 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4441 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4501 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4561 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4621 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4681 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4741 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4801 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4861 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4921 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4981 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5041 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5101 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5161 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5221 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5281 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5341 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5401 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5461 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5521 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5581 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
