LOCUS       sequence-75141-e        5741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse eIF2C3 cDNA clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pBluescript-mouse eIF2C3
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2870..2881
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3507..3523)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3508..3524)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3553..3573)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3553..3571)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3592..3608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3592..3608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3605..3627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3616..3632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3640..3646,3647..3664,3665..3670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3685..3706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3823..3840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3994..4582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4074..4093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4753..5544,5545..5613))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5376..5395
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5614..5718)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatcctcc
      721 ccagcaaatt gactccattg gaaacataca ttgtatgttg attcactttt catctctttg
      781 tcacggagaa taaacttgga cttgttaagc gaagtacatt gtgcgtaagg tatcttggtg
      841 aatctgtaca gccttggcaa gagcttgtgg atctcgccca ttgctctgtc ctgaaacatg
      901 gcttccttca gcactgtcat gttccttgtc cacaagatga tatctggctc tgaatgccac
      961 caggtgagcg taatatgctg gtgcagggat agaaacagat cttgtacagc gtacgtaagt
     1021 gtgacagagc tggtaagtga gcagctgaag ttcatctgca gtaaagaagt tgtcatccca
     1081 taaaacatga tagtgtgaag gacggctggt accctgtatt ccagcatggc tacagagata
     1141 aaagtcgaat tcatatgggt gagtaatgtc ggtatccacc gtggttccag ctgggatgtt
     1201 gccgcttctt ccaacccttt ctgtcctatc agcacagaac aaccgcgtat gatgtctctt
     1261 ctgaacaaca atgtaggtta ttccaggttg atagtctttt tccaaactga tacaggcttc
     1321 tcgaattgcc agtagctcat aatataatac ctgtctgaac tgtccttctg aaacaccatc
     1381 ccgatagaag attatacgag taggtttgaa tcgggttgat ttataaaatt ggatgagcag
     1441 ttcccggacc atggaggcca agtcctggat gatttcctgt cggggtctct gaactcttac
     1501 tgtggcacag tatctgcttg gatgggcatc catactacct acaacagcag caatggaggg
     1561 cttttttccg tcaccagcag gcgggtgtgt gacatctgcg cccaggaaga tcactggttg
     1621 ctggaacaca gaaggtcttt gatgaggtac aagaatatta ttgattcctc caagtttaac
     1681 attgatcttt aggcacaagt ttgaaagagt ttgaggagat gtttttatta cattcttgac
     1741 ttgtacacac tgtgtggcca tacccaaaag tgtatctccc acacgtttca cttccgcata
     1801 cactggtgtc ttcccgggca ggatgacgat aatgagctgc agtcctgagt aggtgttctt
     1861 gagatgccgg aacatgggct ccacgctgtc tgccccctgt gcgtatttgc agaagcatgg
     1921 ctgaccctgg atgggcatcc ctgcatcctt ggaaatcttc cgaagctggt ctgtgaaacc
     1981 ttttagtatt tcttctctgc actgcctctg tgtggcaaag caagcaatag cccacatttt
     2041 gatttcgact cctgtgtgga actgttttcc tcgcatgtcc catactccgt ggctgggtgt
     2101 ggctactgtc cggttccgtc ctccgtactg gagcataggt gctggaagta cacgtcctgt
     2161 cacatgggcc atttcatccc gcactttaag ctgaaactcc tgaacaaatg gatctgtttc
     2221 ataatttgca cttcttacca atctgctaat ttcctcttgt ctatctggtg cagatcttgc
     2281 tgttgccttt atcattgtag aagtctgatt gtccgttagt ttcttgatac acctttgccc
     2341 agccacaata ttacagactt ctagtggcag gtatgtatgc ttctgctcct gccccacttg
     2401 caggcaggga aggtgtgggt acttcagctg cagcgtgtac ttttctctga aatactgagc
     2461 cacagtcctc tccacagttt ggccattttc tagctgtaaa ggaaaggttt ggtggctggc
     2521 aggccttctc gttacattac aaacacggta tttccgtctc attgttccac aatgagtcac
     2581 ttcaactttc agacctttta tctccttagt gaattttacc cgatgagaat cagtcagggg
     2641 tctgggttgt tcatcaatgt tgtgaatgtc aagaacttca cacatgaact gaattacagg
     2701 ttgtgctttg taaaaggcag tggcagaaac atcaatattt agcatcattt tccacatggc
     2761 aggccgaaca gactgatgga atccaaacca tacttccctg ccccctccca gagggtggtc
     2821 atatccttct ggagctgaga aaaaggaacg ccccacaggc gtatatttca tagagggcag
     2881 atgtcggagc accacgtcaa cggcatggac ggggttagtg ctgacaggct tgtctaattc
     2941 cagtggctca ggcaaagtcc ctccagccag tgcttcatgt agtaggtgcc agctgactcg
     3001 agacacaaat ttgactgaca ccttgaaagg tcgatctttt ccaccttccc caggtaatgt
     3061 aacatctaaa tctaccccag tggttgccac aggaagtgga ttggctgtat aaaggcttct
     3121 ttttccatca taaactggtc tacggtctcc aaatatagtc actttaaaat gctgaacctt
     3181 tgaatcaact acttctctgt tcactcttcg aggacacttg tctggtttga tgtcaacttc
     3241 atagaggtag acatcaatct ttgggatttc aacttgaaaa cagttagcca acagtttaat
     3301 gggtttgccc atagtgccgt acccaggtct tctgggcacg atgaataggg gctgggcccc
     3361 aatgggtcct gcggagccga tttccattca tggagctggg gggagtgctt cacgagagaa
     3421 gggtgccacc acagaggcga ggagcggacg ccctcggaaa gctccgggca caagaggcac
     3481 agtcgggaga gacagatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc
     3541 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     3601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5701 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
