LOCUS       sequence-75143-e        5619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse eIF2C4 cDNA clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pBluescript-mouse eIF2C4
FEATURES             Location/Qualifiers
     source          1..5619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      724..733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1203..1214)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3385..3401)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3386..3402)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3431..3451)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3431..3449)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3470..3486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3470..3486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3483..3505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3494..3510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3518..3524,3525..3542,3543..3548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3563..3584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3701..3718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3872..4460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3952..3971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4631..5422,5423..5491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5254..5273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5492..5596)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatccccc
      721 gccgccgcca tggaggcgct gggacccgga cctccagcca gcctcttcca gccacctcgg
      781 cgtcctggcc ttggaaccgt tgggaaacca attcgactgt tagccaatca ttttcaggtt
      841 cagatcccta aaatagatgt gtatcactat gatgtggata ttaaaccaga aaaacggcct
      901 cgtagagtca acagggaggt ggtagacact atggtgcggc acttcaagat gcagatattt
      961 ggagatcggc agcctggcta cgatggcaaa agaaacatgt acacggcaca cccactgcca
     1021 atcggacggg ataggattga tatggaggtg accctcccag gtgagggtaa agaccaaact
     1081 ttcaaagtgt ctgtgcagtg ggtgtcagtt gcgagccttc agctgctttt agaagcttta
     1141 gcaggacacc tgaatgaagt cccagatgac tcagtacaag cacttgatgt gattacaagg
     1201 catctcccct ctatgaggta cactccagta ggccggtctt ttttctcacc tcctgaaggt
     1261 tattaccacc ctctgggagg gggcagagag gtttggtttg gctttcatca gtctgtgaga
     1321 cctgccatgt ggaatatgat gctcaatatt gatgtatctg caactgcttt ctaccgggct
     1381 caacctatca ttgagttcat gtgtgaggtt ttagacattc agaacatcaa tgagcagaca
     1441 aaacctctaa cagactccca gcgtgtcaag tttaccaaag aaattagggg tcttaaggtt
     1501 gaggtgactc actgtggaca gatgaaacga aaatatcgag tttgtaatgt gacaagacgg
     1561 ccagccagtc accaaacctt ccctttgcag ctagagaatg gccaagctat ggaatgtaca
     1621 gtggctcagt attttaagca gaagtatagt ctgcagctga aacaccccca ccttccctgc
     1681 cttcaagtgg ggcaagagca aaagcatacc tacctaccac ttgaggtctg taatatagtg
     1741 gcaggacaga gatgtataaa gaagctcaca gacaatcaga catccaccat gatcaaagcc
     1801 acggcaagat ctgctcctga cagacaggag gaaatcagta gactggtgaa gagcaacagc
     1861 atggtgggtg gacctgaccc atacctgaag gaatttggga ttgttgtgca caacgagatg
     1921 acagagctca caggcagggt gcttccggct ccaatgctgc agtatggagg ccggaataaa
     1981 acagtagcca cacccagcca gggtgtctgg gacatgcgag gaaaacagtt ttatgctggc
     2041 attgaaatta aagtatgggc agtggcttgt tttgcgcctc agaaacaatg tagggaagat
     2101 ttgctaaaga gtttcactga ccaacttcgt aaaatctcca aggatgcagg gatgcccatc
     2161 cagggtcagc catgtttctg caagtatgct caaggtgcag acagcgtgga gcccatgttt
     2221 aaacatctga aaatgacata cgtgggcctg cagctaatag tggtgatttt gcctgggaag
     2281 acaccagtat atgcggaggt gaagcgtgtt ggagataccc ttctaggtat ggccacacag
     2341 tgtgtccaga tcaaaaacgt tgtgaagacc tcgccccaga ccctttccaa cctttgcctg
     2401 aagatgaacg caaagctggg agggattaac aatgtgcctg tgcctcacca aaggccctca
     2461 gtgttccagc agcctgtcat ctttctggga gcagatgtca ctcatccgcc tgctggggac
     2521 gggaagaagc cttctattgc agccgtggtg ggcagcatgg acggccatcc cagccggtac
     2581 tgtgccacag tctgggtaca gacatctcga caggagatcg cccaggagct cctctacagc
     2641 caggaggtcg tgcaggacct gacgagcatg gctcgggagc tgctgattca attctataag
     2701 tccacgcgct tcaagcccac acgcatcatc tactaccgcg gaggggtatc cgagggacag
     2761 atgaagcagg tagcttggcc ggagctaata gcaattcgaa aggcatgtat aagcttggag
     2821 gaagactatc ggccgggaat aacttacatt gtggtgcaaa agaggcacca cacacgactc
     2881 ttctgtgcag ataaaatgga aagggtgggg aaaagtggca atgtcccagc aggcaccaca
     2941 gtggatagca ccgtcacaca cccgtctgag tttgactttt acctctgtag tcatgcagga
     3001 attcagggaa ccagccgtcc ttcacattac caggtcttgt gggatgacaa ctgcttcact
     3061 gcagatgaac tccagctact gacttaccag ctctgtcaca cctacgtgag gtgcacacgc
     3121 tccgtctcca ttcctgcccc tgcatattat gcccggcttg tggcattccg ggcaaggtat
     3181 catttggtgg ataaagatca tgacagtgcg gaaggcagtc acgtgtcagg acagagcaac
     3241 ggccgggatc ctcaggcctt ggctaaggct gtgcagatcc accatgatac ccagcacact
     3301 atgtattttg cctgagagtc ttggaaaaga actcaacaaa tttggcatct cacgtatcct
     3361 caaaatgttt ccgatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
     3421 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     3481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5581 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
