LOCUS       sequence-75144-e        8863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse Dicer cDNA clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pBluescript-mouse Dicer
FEATURES             Location/Qualifiers
     source          1..8863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6629..6645)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6630..6646)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6675..6695)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6675..6693)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6714..6730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6714..6730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6727..6749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6738..6754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6762..6768,6769..6786,6787..6792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6807..6828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6945..6962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7116..7704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7196..7215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7875..8666,8667..8735))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8498..8517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8736..8840)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgataaatt
      721 ctgccttcag ttttcctgtt ctgtgtcagc ccgaaccatc cttgtgccgt ggagctgtgg
      781 ttctggtccg ctgtcttgcc gcctgtgagt ccggaagctt cagctgttag gaacctgagg
      841 ctggttagct ttgaggctgc ggagggccct tcgtgctgca gcagacttgg cgatcctgta
      901 gctccggcca acacctttaa atttcccctt tcccaccact tccacggtga ctctgacctt
      961 tccatcataa gttctctccg ctgggctaaa ctcggcagtt tctggttcca tctcgagcaa
     1021 ttctctcact ggagaacgag gcacatttgc agaaaacttt tctattagag gctgcatcat
     1081 cggatagtac acctgccaga ccacctccag agacatccca ctgtccatat aaatggcacc
     1141 agcaagagac tcaaaaatgt cccccatggc cttggggact tcgatatcct cttctttctc
     1201 ctcatcctcc tcggatctcc taagctcaga gtccattcct tgcatttcgt tcttctcaag
     1261 ctgaaacttc acaaagtcat caataacatg aaagagctca ggagagacag ctttaaagta
     1321 cttgtggtag tcatacttca cagccaatga tgcaaagatg gtgttattga caagggcaga
     1381 gcgcaagtca gtcaagaccc ctggagaatg ctgccgtggg tcttcataaa ggtgcttggt
     1441 tatgaggtag tccaaaatcg catctcccag gaattctaag cgctggtaac aatcagtgat
     1501 agtgttgtag tggtaggagg cgtgtgtaaa agcctgcaga agataagcct tattcttgaa
     1561 tctatagtta attttcttct caaaagtttc aaacccagat atcaggtggt tgagggtttt
     1621 ctctgcgtct ggatggtcaa acatgcacct cggtgggatc ttcaggcagc cgtactccaa
     1681 gtctttccct gcagaggagc gtgggccaac tggagaggca cagctccctg aaaggctctt
     1741 ttgttggctg cttccgttct cctgagcagg gtccaaggcc ttctcccggc tggtcctttt
     1801 gattactggg agcaccttca gccccagcga acagaggaag agctgagcag ccctctcgcc
     1861 gcagctggtt aagtagcagc ccagcagggc ctccacacag tccgctatgc tcttgtcagc
     1921 aatgcactgc tccgtgtgca agtcgtaaga aatggactgc ttccctgtgt cgacgccaca
     1981 gttctcttct gatggattcc agaaacctac cacaaagtca tcctcttcaa cggctttgct
     2041 aggatccaga tagcacatcg catcccaaga gctgtaatca aaatcctcaa ggcccgaggc
     2101 aaacggcata ctgcccaagg aggccttctt gggcatcttc cattcgtatg cagagtcgga
     2161 ggctgagaag ggcgacagag agattttcct cacgaaggcg cccgagccca tgagcatgct
     2221 gtctataaac tggatatgct cctggtcata ctccaggaag tcgtcctcat cctcggcctc
     2281 ctccttgggg gccctccacg tcaggtcctc ctcctcctca caggcctcac ccagtttgcc
     2341 attagccagc aagcagtctt ttgtcatttc atccttttcc cacttctctg agttactttt
     2401 gtcttggttt accacataac caggaggaag ccaattcaca ggaggatcga atatcgacac
     2461 caccatgcgg ctgggcagtc ccttcttctt gccaaggcga tacaggttac agttgctgac
     2521 ctttttgctt ctcatatatg aaaggcggcc ttcatgagca tcagggtacg tgcaaaacag
     2581 atatgtggtg atagcatgct ttaaaaagga gtcgccaagc atctcaagcc gctccaggtt
     2641 aaacccatca ctggcatttg acagtgtcaa agcctggaga atcagtcccg gattggggcc
     2701 aagagtcctt gaggagtacc caacagaagg gctctgctca gagtccatcc tgtccttgag
     2761 tgctttaaca gcattcatca tggcaggcat cgtggacacc gcggggcttc catctgaggt
     2821 agatgtgtta gcgttcccat caaggtacgt attactcagt agaggacatt cattgctggg
     2881 cttgggctgg ttctcataat tgtacaaatt ctgaatggga tatgaggtag ttggttgtac
     2941 aggtatttcc tgcttgaagt aattcagctg atttccttgg caaaagtctc tgttaactaa
     3001 atcataactg ccattggcaa ggtttttatt gtaagaaaga ccattaattg ctgtaagatc
     3061 cgttgaaacc tcactttgga gcttagcagg tgactcagcg ggcaaccttt tgcagttcac
     3121 agacatttgg tcatggtttt ctaaggaggg tctagtagca ccttgatgtg cagcatgttc
     3181 agggactaca gttgtgctgt gcttacagta attatcactc tcagccaacg aggagttaca
     3241 gcttgagatg aaagacttgc tatcaataga ttttttccac ccgaagtcta agttagggta
     3301 tccaaaatcc acgggaagtg atctgacacc cacaccagca tcgctggctg tctgagctct
     3361 tagttcctct gcagtcagaa ggcagtgaag gcgatacagt atgctgggga gacaaaccgc
     3421 ttttctccac agtgatgctg ggattggatg tatagcacag agttccggaa ccaggatctg
     3481 tttgttctgc agactttccc atttggcctt cctcttctca gcactgctta gaggaagtgc
     3541 tttccccttc tgattcaaat gccgaggtgt caaaaggttc agtctggaag atgcgtggtc
     3601 aacatccagc agtggctggt tgagattggt caggtcaagg ttatactttg ttttataata
     3661 ttctgcaaaa gtttcacatt caggggaagg aaatttactg agtggggtaa gatccgtata
     3721 tacatcagct acataaaatc gatgaggctg atcaaaattg cggtatcttg ggatgatcac
     3781 tgcatcttgg taatcttcta acttaaagac aaacggtgtt tcctttgaat acttggtgct
     3841 aggaatgcct atccgagctt ccgacttctc aatatcctcc atgaatttaa agtcaatgtc
     3901 caaagtgccg gagtcattaa caacattaag aggtagaaca cagtaggctg actccgcgcc
     3961 tgtaggttta aactccaggg caggcttctc aagccggaga atatgtgaga atatgtactg
     4021 gtgcaatctt gtaatcagct cgagcatttg ttgagacaat gtgaaaccag acttcttcag
     4081 ctcgatggat atggtgacct ctccagagcg tgtatacaca ggaaagtgag gaatctgagg
     4141 tatgggtttg gctgtcagga tcccaaagca tcttgtggtg tcctcaggtg ggtagagctt
     4201 ccgccgcctg aagttgagtt catcagggag gggggtagtt aagaccattc ctatcacata
     4261 caggtaacaa ggctggtcgg gtttggggta actctccctc aagcactctg gaattgcttt
     4321 ggggtagcac tgccgtcgtt ttgtggaccc tggtctcccg ggaacactgg tctcctcctc
     4381 gtcatgtaag tccagctctt cttcatattt aacagtctct ttcccaactg gcatcaagtg
     4441 ttcatccagt tcaccaattt tgtgcagttt ttcacagcaa atgagagcta ccactctttc
     4501 agccaaccgt acactgtcca tcggtggacc aacaatggag gctcgaagag gtgagttaat
     4561 cggaagataa agagttgaat aaaatgtacc atcaggcaat tctcgggttc tacatttggg
     4621 agctagatgc gtaaacggat cacttggtaa tcgagcacaa tatctgttga tgtgtccaat
     4681 ggcagtgttg attgtgactc gaggaccccc atcatcaggc ctcagcacat atggcgggaa
     4741 agtatcctca tcatctacac cagcatggac gtccgcctca gcaccatccg ctgacttcga
     4801 acacttgttc cttaagatct tttcaatagc tttgtaggtc ttgaggtctt cttcaaagct
     4861 ctttatcttg tctgtatctg ctaacattac gtaattagag atcggcgctc gtgcccgtcc
     4921 tttagactgg acatatgatc gatactctgt aggcaaatca aaacgaacca ccaaattgca
     4981 cttgggaata tccacaccct cttctacaac acttgttgca atgagcaggt tggtctcatg
     5041 agctcgaaat ttcctaagta cctcctcctg ctttctgaac tccgcctcca tctgtttgct
     5101 gcgaggctgg ttctttccaa tgccgtgtcc agttataaag ttgctgctga tataagccag
     5161 ctctggatct tgtttgccag cttcctttat caatctgttt aggacaactg ctgtgtatct
     5221 tctttccaca aaaataatcc cacataaaat attggtaaac ggagaaggaa aatttgtctc
     5281 tggcttttct ttttcttcaa tttcttcgtc atcatcgtca tcctcggaat cactccagga
     5341 cacgtaatta tcctgattcc tgttattgta ccactcaaca ctttcaaact gctgtcgctc
     5401 atagggcttg tatttgcgca ggatctcgag cagtttcatg actttgggag ttacatactt
     5461 caggtcaagt gaggcaggtg agaagtactc ttcacacagg gcgtgtattt tccttaacaa
     5521 ggtgtctgta aacaatagga acttcctgtg tagctcctct tgctcatgtt tgatgtactt
     5581 ctgaagttct cttaccatca tcccagctac tttatctgca caccagggtc ccagaacaac
     5641 caacactgca cggcagtctg agaggatttg tttggaaatc aaagtagaat ctctttcctt
     5701 tgaatgtaca gctacattac aatcattgat aaaatcaagt gctgcttcta actccatcag
     5761 caatctctca tacagcccac ttctgtcagt aaacggtcca cagtctacca caatctcaca
     5821 aggctgagaa gtgtacctgt ccaggaccac caagtcagtc gccgtctcag cgtcactcct
     5881 gagaattcgc tccagtttct gaatcttctc ttccaattcc tctggatcac atttcccatt
     5941 taaaatggaa gcagttagtc ccaaaattcg agggcatgat ggacaactct cacagagctt
     6001 cataatttca cgataggggt ggtctagaat cgcaagatga cactcatcaa acaccaaaag
     6061 gttaatgtct ggcagtgata agtaaccatt tttcaaaaca gtcaaggcga catagcaagt
     6121 cataatgaga acctggtgct tagtaaactc ttggctccat ctctcttttg tccaagatgc
     6181 atttacttct aggtctgagt actctccaac cttgagatcc gagtgagttc tgacggctga
     6241 cacttgttga gcaacctggt ttgcagagtt gacgaggaac acggtccttt ttgcatgcgg
     6301 gttgaggtcg cccctgatct gatgggccag ctctttggtg agcaggaccg cgatgaacgt
     6361 cttccctgag ccagtgttca agcacacaat tgtgttatgg tccagagctg cttccagcag
     6421 ctcaacctgg tattttcttg gcgtataaat gttatcatga attgcttctt gttgccatgg
     6481 cagtccaaag aaaggaccca ttggtgagga agcaggggtc atgagctgca ggcctgccat
     6541 gctgaggggc tgcaaagcag ggcttttcaa tcatccagtg tttctttcat tgcattttgt
     6601 tctagcacag cttactgatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
     6661 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     6721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8161 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8461 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
