LOCUS       sequence-75148-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trasgenically expresses tandem tdtomato fluorescent protein in T. 
            cruzi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson 
            LJ, Xu D, Boehlke CL, Tarleton RL
  TITLE     In vitro and in vivo high-throughput assays for the testing of 
            anti-Trypanosoma cruzi compounds.
  JOURNAL   PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 
            10.1371/journal.pntd.0000740.
  PUBMED    20644616
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2010-07-13"; volume: "4(7):e740. doi"; pages: " 
            10.1371/journal.pntd.0000740"
COMMENT     Sequence Label: pTrex-Neo-tdTomato
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(822..841)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcgagctcta agtcgacctc gagccattta cgactccaag gcaacgctgc agaacaacct
       61 gccgaaggag cgccgcttct tcaagattgt gtcgtggtac gacaacgagt ggggatactc
      121 ccaccgcgtg gtggaccttg tacgccacat ggcctcgaag gatcgttcgg caaggttgta
      181 ggcgtggcga tgacttcagg tctttctttt gcgaataggg atcttataat acacgatgcg
      241 tgtcccgtga tgatcgttac cggtgctgcc acgatccaat tgacacagcg tcaagagcaa
      301 aacaatttta cttttccctt taaggacaac aacaaaaaaa tatataactt ttttttcttt
      361 tttttttttt ttgaaattat atttatggtc atctttggga aacaaaaagc agcaatttaa
      421 tgatgcggaa ggatgagtga aataatgttt aatcaatgta cgaggatttg gggtattgca
      481 aggaaaatgt agatgattta attgggtgtg tgatgcagct tgtggtaatt tttgctcact
      541 tccctttttg ccacatcttt ttagtttttc tgcttttctt tccccattat tccacttgtc
      601 tctcttttcc cacgtttcct gcacgaatgc agaaagtgat atttttactt tgaaagccat
      661 ctaccaacaa caattacatt gaacagaatt tgggaattgc gaattaattc ctgcagcccc
      721 gcgtgtggcc tcgaacaccg agcgaccctg cagccaatat gggatcggcc attgaacaag
      781 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
      841 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
      901 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
      961 cgcggctatc g
//
