LOCUS       sequence-75150-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transgenically expresses firefly luciferase protein in T. cruzi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson 
            LJ, Xu D, Boehlke CL, Tarleton RL
  TITLE     In vitro and in vivo high-throughput assays for the testing of 
            anti-Trypanosoma cruzi compounds.
  JOURNAL   PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 
            10.1371/journal.pntd.0000740.
  PUBMED    20644616
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2010-07-13"; volume: "4(7):e740. doi"; pages: " 
            10.1371/journal.pntd.0000740"
COMMENT     Sequence Label: pLew90-beta-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(95..119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(282..302)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      376..423
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      508..555
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
       61 aaggccaaga agggcggaaa gatcgccgtg taagacccag ctttcttgta caaagtgggg
      121 tcttaaggat cctgcccatt tggcttttcc cttgtctcgt gtcttttccg tggaaaggtt
      181 cccggagtaa tctgatggca cagcagggag gtgcgcctgc aggttggtta ggaagggggg
      241 atgatgtaaa agaagaaaat ggggggattc gagcccgggc acaactttat tatacatagt
      301 tgataattca ctggccgtgg gcccttcgaa ggtaccgagc tcttaattaa aagcttccga
      361 gccgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      421 ttgctgaaag gagaactata tccggatagg gctgctaaca aagcccgaaa ggaagctgag
      481 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      541 ttgaggggtt ttttgctgaa aggaggaact atatccggct acgcgttcta gacccgggga
      601 tatcactagt gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
      661 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
      721 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
      781 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
      841 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
      901 gtgtcacgtt gcaagacctg cctgaaaccg aa
//
