LOCUS       sequence-75155-e        4782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pLux-LmrA
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     86..107
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(601..620)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             612..1427
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             612..1427
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(685..704)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1544..1561
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      2135..2723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2624..2643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      4662..4733
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4749..4776
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
       61 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      121 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
      181 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatttc actgacaccc
      241 tcatcagtgc caacatagta agccagtata cactccgcta gcgctgaggt ctgcctcgtg
      301 aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga
      361 gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt
      421 gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag
      481 caaaagttcg atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg
      541 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
      601 caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt
      661 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
      721 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
      781 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
      841 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
      901 tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat cctgattcag
      961 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     1021 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     1081 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     1141 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     1201 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     1261 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     1321 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     1381 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     1441 attggttgta acactggcag agcattacgc tgacttgacg ggactcgaga ttaccgcctt
     1501 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1561 ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga
     1621 caccttgccc gtttttttgc cggactgcag caatggcaac aacgttgcgc aaactattaa
     1681 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1741 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1801 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1861 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1921 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1981 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2281 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2881 aggtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     2941 cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg
     3001 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccctcgagc cgtcaattgt
     3061 ctgattcgtt accaattatt aatttttaaa gtatgggcaa tcaattgctc ctgttaaaat
     3121 tgctttagaa atactttggc agcggtttgt tgtattgagt ttcatttgcg cattggttaa
     3181 atggaaagtg acagtacgct cactgcaacc taatattttt gaaatatccc aagagctttt
     3241 tccttcgcat gcccacgcta aacattcttt ttctcttttg gttaaatcgt tgtttgattt
     3301 attatttgct atatttattt ttcgataatt atcaactaga gaaggaacaa ttaatggtat
     3361 gttcatacac gcatgtaaaa ataaactatc tatatagttg tctttttctg aatgtgcaaa
     3421 actaagcatt ccgaagccat tgttagccgt atgaataggg aaactaaacc cagtgataag
     3481 acctgatgtt ttcgcttctt taattacatt tggagatttt ttatttacag cattgttttc
     3541 aaatatattc caattaattg gtgaatgatt ggagttagaa taatctacta taggatcata
     3601 ttttattaaa ttagcgtcat cataatattg cctccatttt ttagggtaat tatctaggat
     3661 tgaaatatca gatttaacca tagaatgagg ataaatgatc gcgagtaaat aatattcaca
     3721 atgtaccatt ttagtcatat cagataagca ttgattaata tcattattgc ttctacaagc
     3781 tttaatttta ttaattattc tgtatgtgtc gtcggcattt atgtttttca tcgaggcctt
     3841 tcctgtgtga attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     3901 gaaaggtttt gcgccattcg atggcgcgcc gcttcgtcag gccacatagc tttcttgttc
     3961 tgatcggaac gatcgttggc tgacctgtag gatcgtacag gtttacgcaa gaaaatggtt
     4021 tgttatagtc gaataaacta tggactatgt tttcacacag gaaagggctc gatgagctat
     4081 ggtgatagcc gtgaaaaaat tctgagcgca gcaacccgtc tgtttcagct gcagggttat
     4141 tatggcaccg gtctgaatca gattatcaaa gaaagcggtg caccgaaagg tagcctgtat
     4201 tatcattttc cgggtggtaa agaacagctg gcaattgaag cagtgaacga aatgaaagaa
     4261 tatatccgcc agaaaatcgc cgattgtatg gaagcatgta ccgatccggc agaaggtatt
     4321 caggcatttc tgaaagaact gagctgtcag tttagctgta ccgaagatat tgaaggtctg
     4381 ccggttggtc tgctggcagc agaaaccagc ctgaaaagcg aaccgctgcg tgaagcatgt
     4441 catgaagcat ataaagaatg ggccagcgtg tatgaagaaa aactgcgtca gaccggttgt
     4501 agcgaaagcc gtgcaaaaga agcaagcacc gttgttaatg caatgattga aggtggtatt
     4561 ctgctgagcc tgaccgcaaa aaatagcaca ccgctgctgc atattagcag ctgtattccg
     4621 gatctgctga aacgttaact agggcccata cccccaggca tcaaataaaa cgaaaggctc
     4681 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     4741 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//
