LOCUS       sequence-75156-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic mRFP tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-mRFP-ura4MX6
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..748)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(71..748)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(133..156)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(331..350)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     600..618
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(809..827)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(810..827)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(908..927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 cttcgaattc ttaggcgccg gtggagtggc ggccctcggc gcgctcgtac tgttccacga
      121 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttgat gtcggtcttg taggcgccgg
      181 gcagctgcac gggcttcttg gccatgtagg tggtcttgac ctcggcgtcg tagtggccgc
      241 cgtccttcag cttcagcctc atcttgatct cgcccttcag ggcgccgtcc tcggggtaca
      301 tccgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg
      361 ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt
      421 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
      481 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
      541 tggagccgta ctggaactga ggggacagga tgtcccaggc gaagggcagg gggccgccct
      601 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
      661 cgatctcgaa ctcgtggccg ttcacggagc cctccatgcg caccttgaag cgcatgaact
      721 ccttgatgac gtcctcggag gaggccatgt taattaaccc ggggatccgt cgacctgcag
      781 cgtacgaagc ttcagctggc ggccgcgttc tatagtgtca cctaaatcgt atgtgtatga
      841 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtcc atatggtgca
      901 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccga
//
