LOCUS       sequence-75161-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AND gate in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pAND
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      46..117
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      133..160
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      872..943
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      959..985
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ccagcatctg ccgaccgatt aatcgtaagg cgagccccca ggcatcaaat aaaacgaaag
       61 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      121 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatagctt tactccaccg
      181 ttggcttttt tccctatcag tgatagagat tgacatccct atcagtgata gagataatga
      241 gcacgccatg ccattggctt ttgatagagg acaactacta gatgaacctg aaagataaaa
      301 ttctgggcgt tgccaaagaa ctgtttatca aaaatggcta taacgcaacc accaccggtg
      361 aaattgttaa actgagcgaa agcagcaaag gcaatctgta ttatcacttt aaaaccaaag
      421 agaacctgtt tctggaaatc ctgaacatcg aagaaagcaa atggcaagag cagtggaaaa
      481 aagaacaaat caaatgcaaa accaaccgcg agaaattcta tctgtataat gaactgagcc
      541 tgaccaccga atattactat ccgctgcaga atgccatcat cgagttttat accgagtact
      601 ataaaaccaa cagcatcaac gagaaaatga acaaactgga aaacaaatac atcgatgcct
      661 accacgtgat ctttaaagaa ggtaatctga acggcgaatg gtgcattaat gatgttaatg
      721 ccgtgagcaa aattgcagca aatgccgtta atggcattgt tacctttacc catgagcaga
      781 atatcaacga acgcattaaa ctgatgaaca aattcagcca gatctttctg aatggcctga
      841 gcaaataagg actgcgacgt tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      961 ctcaccttcg ggtgggcctt tctgc
//
