LOCUS       sequence-75163-e        4710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express DreO recombinase
            in neurons from the synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: AAV phSyn1(S)-DreO-bGHpA
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        177..624
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             671..691
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             692..1714
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     primer_bind     complement(1737..1754)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1743..1950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1973..2113
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1973..2102
                     /label=AAV2 ITR
     rep_origin      2188..2643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2275..2294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2485..2506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2660..2679)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2779..2801
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2839..2857)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2925..3029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3030..3098,3099..3890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3248..3267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4061..4649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4550..4569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctgca gagggccctg cgtatgagtg
      181 caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg cctacctgac gaccgacccc
      241 gacccactgg acaagcaccc aacccccatt ccccaaattg cgcatcccct atcagagagg
      301 gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca
      361 gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg
      421 acgtcactcg ccggtccccc gcaaactccc cttcccggcc accttggtcg cgtccgcgcc
      481 gccgccggcc cagccggacc gcaccacgcg aggcgcgaga taggggggca cgggcgcgac
      541 catctgcgct gcggcgccgg cgactcagcg ctgcctcagt ctgcggtggg cagcggagga
      601 gtcgtgtcgt gcctgagagc gcagacgcgt ggacggtacc cttcaggtcg agaaatcgat
      661 tgccaccatg cctaagaaga agaggaaggt ttctgagctg attattagtg gttcatctgg
      721 tggattcctg cgaaacatcg gcaaagagta tcaggaggcc gctgaaaact tcatgaggtt
      781 tatgaatgac cagggggcgt acgctcctaa cactttgagg gatttgaggt tggtctttca
      841 tagctgggcc agatggtgcc atgctcggca gcttgcatgg tttccaatta gtcctgaaat
      901 ggcacgcgaa tactttcttc agttgcacga tgcagacctg gcctccacta ccatcgacaa
      961 gcactatgct atgcttaata tgcttctgtc ccactgcgga ctgccaccct tgtccgacga
     1021 caagtcagtg agtcttgcca tgagaagaat tagaagagaa gccgcaaccg aaaagggtga
     1081 gaggacagga caggcaatcc ccctgcgctg ggacgacctg aagctgctgg atgtgctgct
     1141 cagcaggagc gagcggctgg tcgacctgcg caacagggct ttcctgttcg tagcctataa
     1201 caccctcatg agaatgtctg aaatatcacg catcagggtt ggggacttgg atcagacagg
     1261 agacacagtg accctgcaca tcagtcacac taagacaatc accacagctg cgggccttga
     1321 caaagtgctc tcccggcgaa ccacagcagt gctcaatgac tggctggacg tcagtgggct
     1381 tagagaacat ccagacgctg tgctcttccc acctatacac cggtcaaaca aagcccgcat
     1441 tactaccacg cccctgaccg cccctgccat ggagaagatt ttcagtgatg cctgggtgct
     1501 gctgaacaaa cgggacgcca cccccaataa agggaggtat aggacctgga ccggccattc
     1561 cgccagggtg ggtgccgcaa tagacatggc cgagaaacag gtgtctatgg tcgagattat
     1621 gcaggaaggg acatggaaga agcctgaaac actgatgcgg tatctcagaa ggggcggagt
     1681 gtccgtggga gccaattctc gactgatgga tagctaagga tccgctagca gatctgcctc
     1741 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     1801 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     1861 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     1921 ttgggaagac aatagcaggc atgctgggga cacgtgcgga ccgagcggcc gcaggaaccc
     1981 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     2041 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     2101 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2161 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     2221 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2281 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2341 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2401 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2461 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2521 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2581 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     2641 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     2701 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2761 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2821 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2881 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2941 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3001 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3061 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3121 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3181 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3241 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3301 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3361 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3421 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3481 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3541 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3601 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3661 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3721 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3781 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3841 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3901 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3961 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4201 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4681 tggccttttg ctggcctttt gctcacatgt
//
