LOCUS       sequence-75164-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  single copy recombinase yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Ansari A, Cheng TH, Gartenberg MR
  TITLE     Isolation of selected chromatin fragments from yeast by 
            site-specific recombination in vivo.
  JOURNAL   Methods. 1999 Feb;17(2):104-11.
  PUBMED    10075889
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "1999-02"; volume: "17(2)"; pages: "104-11"
COMMENT     Sequence Label: pRS415-recR
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..48
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     33..49
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..99)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(83..99)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(124..454)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    337..454
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat
       61 atcgaattcc tgcagcccgg gggatccact agttctagag cggccgctaa ttccttgaat
      121 tttcaaaaat tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca
      181 tggcattacc accatataca tatccatata catatccata tctaatctta cttatatgtt
      241 gtggaaatgt aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca
      301 gtaatacgct taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt
      361 gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct
      421 gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc
      481 ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa
      541 cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg
      601 gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact
      661 gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat
      721 gtaataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg t
//
