LOCUS       sequence-75165-e        8818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human MICU1 with Flag tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi 
            ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O,
            Mootha VK
  TITLE     EMRE is an Essential Component of the Mitochondrial Calcium 
            Uniporter Complex.
  JOURNAL   Science. 2013 Nov 14.
  PUBMED    24231807
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Nov 14."
COMMENT     Sequence Label: pLYS1-MICU1-Flag
FEATURES             Location/Qualifiers
     source          1..8818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1827..1850
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1901..2018
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2067..2577
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2496..2514
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2599..3198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2599..2618)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3095..3115
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3326..3559
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3631..3752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3668..3687)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3722..3741
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3792..3927
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3947..3966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3948..3966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3976..3993)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3976..3992)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3985..4007)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4134..4589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4221..4240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4431..4452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4615..4719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4720..4788,4789..5580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4938..4957)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5751..6339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6240..6259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6493..6510
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6627..6648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6663..6668,6669..6686,6687..6693)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6701..6717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6706..6728
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6725..6741
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6725..6741
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6760..6780
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6762..6780
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6808..7034
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7035..7215
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7262..7387
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7880..8113
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8298..8342
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8640..8660
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcatgtttcg tctgaactca ctttctgctt tggcagaact ggctgtgggt tctcgatggt
      421 accatggagg atcacagccc atccagatcc ggcgaagact aatgatggtg gctttcctgg
      481 gagcatctgc agtaactgca agtactggtc ttttgtggaa gagggcccat gcagaatctc
      541 caccatgtgt agacaaccta aaaagtgaca tcggtgataa agggaagaat aaagatgaag
      601 gggatgtttg taaccatgag aaaaagactg cagatcttgc ccctcaccca gaagagaaaa
      661 agaagaaacg ttctggattc agagacagaa aagtgatgga atatgagaat aggattcgag
      721 cctactccac gccagacaaa atcttccgat attttgccac cttgaaagtc atcagtgagc
      781 ctggtgaagc agaagtgttt atgacaccag aagattttgt gcgatccata acacccaatg
      841 aaaaacaacc agaacacttg ggtctggatc aatatataat aaaacgcttt gatggaaaga
      901 aaatttccca ggaacgagaa aaatttgctg atgaaggcag tatattttac acccttggag
      961 aatgtgggct catatccttt tcagactaca ttttcctcac aactgttctt tccactcctc
     1021 agagaaattt tgaaattgcc ttcaagatgt ttgatttgaa tggagatgga gaagtagata
     1081 tggaagaatt tgaacaggtt cagagcatca ttcgctccca aaccagtatg ggtatgcgcc
     1141 acagagatcg tccaactact ggcaacaccc tcaagtctgg cttgtgttca gccctcacaa
     1201 cctacttttt tggagctgat ctgaagggaa agctgacaat caaaaacttc ctcgaatttc
     1261 agcgtaaact gcagcatgat gttctgaagc ttgagtttga acgccatgac cctgtggatg
     1321 ggagaattac tgagaggcag tttggtggca tgctacttgc ctacagtggg gtgcagtcca
     1381 agaagctgac cgccatgcag aggcagctca agaagcactt caaagaagga aagggtctga
     1441 catttcagga ggtggagaac ttctttactt tcctaaagaa cattaatgat gtggacactg
     1501 cattgagttt ttaccatatg gctggagcat ctcttgataa agtgaccatg cagcaggtgg
     1561 ccaggacagt ggctaaagtg gagctctcag accacgtgtg tgatgtggtg tttgcactct
     1621 ttgactgtga tggcaatggc gaactgagca ataaggaatt tgtttccatc atgaagcaac
     1681 ggctgatgag aggcctggaa aagcccaaag acatgggttt cactcgcctc atgcaggcca
     1741 tgtggaaatg tgcacaggaa actgcctggg acttcgcttt acccaaacag cccgggggtg
     1801 gatctggtgg atctggtgga tctatggatt acaaggatga cgatgacaag taaggtaccg
     1861 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1921 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1981 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2041 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
     2101 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     2161 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     2221 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     2281 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     2341 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     2401 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     2461 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     2521 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     2581 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     2641 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     2701 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     2761 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     2821 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     2881 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     2941 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     3001 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     3061 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     3121 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3181 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     3241 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3301 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3361 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3421 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3481 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3541 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     3601 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3661 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3721 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3901 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3961 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4021 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4081 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4141 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4201 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4261 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4321 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4381 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4441 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4501 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4561 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4621 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4681 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4741 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4801 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4861 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4921 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4981 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5041 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5101 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5161 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5221 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5281 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5341 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5401 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5461 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5521 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5581 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5641 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5701 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5761 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5821 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5881 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5941 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6001 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6061 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6121 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6181 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6241 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6301 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6361 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6421 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6481 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6541 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6601 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6661 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6721 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6781 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6841 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6901 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6961 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7021 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7081 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7141 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7201 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7261 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7321 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7381 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7441 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7501 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7561 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7621 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7681 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7741 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7801 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7861 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7921 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7981 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8041 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8101 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8161 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8221 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8281 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8341 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8401 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8461 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8521 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8581 tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg
     8641 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     8701 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     8761 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatca
//
