LOCUS       sequence-75189-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from T to
            D at position 308 and S to D at position 473 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-T308D/S473D
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..64)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtcgacatcg ataagcttac gcgtgaattc atctagaagc gtaatctgga acatcgtatg
       61 ggtaggctgt gccactggct gagtagtcga actgggggaa gtgcggcctc cgctcactgt
      121 ccacacactc catgctgtca tcttgatcag gcggcgtgat ggtgatcatc tgagctgtga
      181 actcctcatc gaaatacctg gtgtcagtct cagaggtgac ctggggcttg aaaggtgggc
      241 tcagcttctt ctcatacaca tcctgccaca cgatgttggc aaagaaccgg tgctgcatga
      301 tctccttggc atcctcagag cccccaccga gcctctgtgt agggtccttc ttgagcagcc
      361 cggagagcag ggacttggcc tcagggccga gtgtgcgcgg gaagcggatc tcctccatga
      421 ggatcagctc gaacagcttc tcgtggtcct ggttgtagaa gggcaggcgg ccacacatca
      481 tctcatacat gaccacgccc agcccccacc agtccactgc acggccgtag tcgttgtcct
      541 ccagcacctc aggggccagg tactccggcg ttccgcagaa gtccttcata gtggcaccat
      601 ccttgatccc ctccttgcac agcccgaagt ccgttatctt gatgtgcccg tccttgtcca
      661 gcatgaggtt ctccagcttc aggtcccggt acaccacgtt cttctcggag tgcaagtagt
      721 ccagggcaga cacaatctcc gcaccataga agcgggcccg gtcctcggag aacacgcgct
      781 ctcgagacag gtggaagaag agctcgcccc cgttggcata ctccatgaca aagcagaggc
      841 ggtcgtgggt ctggaatgag tacttgaggg ccgtaaggaa gggatgccta gagttctgca
      901 ggacacggtt ctcagtaagc gtgtgggcaa cctcatcctt
//
