LOCUS       sequence-75193-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR 
            amplification of the intron/kanMX module for one-step 
            homology-driven genomic disruptions .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
  TITLE     Controlling gene expression in yeast by inducible site-specific 
            recombination.
  JOURNAL   Nucleic Acids Res. 2000 Dec 15;28(24):E108.
  PUBMED    11121495
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2000-12-15"; volume: "28(24)"; pages: "E108"
COMMENT     Sequence Label: pRKO
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(77..274)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ttatacgaag ttatattaag ggttctcgag agctcgtttt cgacactgga tggcggcgtt
       61 agtatcgaat cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta
      121 ctttctgcgc acttaacttc gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa
      181 tcacgctaac atttgattaa aatagaacaa ctacaatata aaaaaactat acaaatgaca
      241 agttcttgaa aacaagaatc tttttattgt cagtactgat tagaaaaact catcgagcat
      301 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      361 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      421 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      481 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      541 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      601 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
      661 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      721 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaa
//
