LOCUS       sequence-75194-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR 
            amplification of the intron/kanMX module for one-step 
            homology-driven genomic disruptions .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
  TITLE     Controlling gene expression in yeast by inducible site-specific 
            recombination.
  JOURNAL   Nucleic Acids Res. 2000 Dec 15;28(24):E108.
  PUBMED    11121495
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2000-12-15"; volume: "28(24)"; pages: "E108"
COMMENT     Sequence Label: pRKO
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(84..281)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gcatacatta tacgaagtta tattaagggt tctcgagagc tcgttttcga cactggatgg
       61 cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat
      121 gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa
      181 atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca
      241 aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattag aaaaactcat
      301 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
      361 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
      421 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
      481 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
      541 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
      601 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
      661 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
      721 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
      781 ggaa
//
