LOCUS       sequence-75195-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR 
            amplification of the intron/kanMX module for one-step 
            homology-driven genomic disruptions .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
  TITLE     Controlling gene expression in yeast by inducible site-specific 
            recombination.
  JOURNAL   Nucleic Acids Res. 2000 Dec 15;28(24):E108.
  PUBMED    11121495
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2000-12-15"; volume: "28(24)"; pages: "E108"
COMMENT     Sequence Label: pRKO
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        116..303
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 agcttggtga gcgctaggag tcactgccag gtatcgtttg aacacggcat tagtcaggga
       61 agtcataaca cagtcctttc ccgcaatttt ctttttctat tactcttggc ctcctctagt
      121 acactctata tttttttatg cctcggtaat gattttcatt tttttttttc cacctagcgg
      181 atgactcttt ttttttctta gcgattggca ttatcacata atgaattata cattatataa
      241 agtaatgtga tttcttcgaa gaatatacta aaaaatgagc aggcaagata aacgaaggca
      301 aagatgacag agcagaaagc cctagtaaag cgtattacaa atgaaaccaa gattcagatt
      361 gcgatctctt taaagggtgg tcccctagcg atagagcact cgatcttccc agaaaaagag
      421 gcagaagcag tagcagaaca ggccacacaa tcgcaagtga ttaacgtcca cacaggtata
      481 gggtttctgg accatatgat acatgctctg gccaagcatt ccggctggtc gctaatcgtt
      541 gagtgcattg gtgacttaca catagacgac catcacacca ctgaagactg cgggattgct
      601 ctcggtcaag cttttaaaga ggccctaggg gccgtgcgtg gagtaaaaag gtttggatca
      661 ggatttgcgc ctttggatga ggcactttcc agagcggtgg tagatctttc gaacaggccg
      721 tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag gagatctctc ttgcgagatg
//
