LOCUS       sequence-75196-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR 
            amplification of the intron/kanMX module for one-step 
            homology-driven genomic disruptions .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
  TITLE     Controlling gene expression in yeast by inducible site-specific 
            recombination.
  JOURNAL   Nucleic Acids Res. 2000 Dec 15;28(24):E108.
  PUBMED    11121495
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2000-12-15"; volume: "28(24)"; pages: "E108"
COMMENT     Sequence Label: pRKO
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..21)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        602..706
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttaggg
       61 tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata
      121 aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga
      181 tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc
      241 tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc
      301 tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt
      361 gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat
      421 ttttttttct tcattccgta actcttctac cttctttatt tactttctaa aatccaaata
      481 caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat
      541 acgaggcgcg tgtaagttac aggcaagcga tccgtcaggt ggcacttttc ggggaaatgt
      601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      721 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      781 agaa
//
