LOCUS       sequence-75197-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR 
            amplification of the intron/kanMX module for one-step 
            homology-driven genomic disruptions .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
  TITLE     Controlling gene expression in yeast by inducible site-specific 
            recombination.
  JOURNAL   Nucleic Acids Res. 2000 Dec 15;28(24):E108.
  PUBMED    11121495
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2000-12-15"; volume: "28(24)"; pages: "E108"
COMMENT     Sequence Label: pRKO
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa
       61 ctagtggatc cggtgattga ttgagcaagc tgggtaataa ctgatataat taaattgaag
      121 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
      181 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
      241 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
      301 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
      361 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
      421 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
      481 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggctaa
      541 acatataata tagcaacaaa aagaatgaag caatcgatgt tagtacatga gacttagtaa
      601 cagtagcaaa taaaagaaga atatcactat cacttatcac gaaaatattt cagttttttt
      661 tttttt
//
