LOCUS       sequence-75200-e        6698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BFP in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Strijbis K, Tafesse FG, Fairn GD, Witte MD, Dougan SK, Watson N, 
            Spooner E, Esteban A, Vyas VK, Fink GR, Grinstein S, Ploegh HL
  TITLE     Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to 
            Dectin1-dependent phagocytosis of Candida albicans in macrophages.
  JOURNAL   PLoS Pathog. 2013 Jun;9(6):e1003446. doi: 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27.
  PUBMED    23825946
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013-06"; volume: "9(6):e1003446. doi"; pages: " 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27"
COMMENT     Sequence Label: pENO1-BFP-NAT
FEATURES             Location/Qualifiers
     source          1..6698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(65..262)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(3858..3876)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3897..3913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3897..3913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3910..3932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3921..3937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3945..3951,3952..3969,3970..3975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3990..4011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4128..4145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4299..4887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4379..4398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5058..5849,5850..5918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5681..5700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5919..6023)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6052..6507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6139..6158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6349..6370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6634..6656
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6648..6665
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6649..6665
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6672..6691
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6672..6690
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtaccgaat tcgagctcgt ttaaacgtcg acactggatg gcggcgttag tatcgaatcg
       61 acagcagtat agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac
      121 ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat
      181 ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa
      241 caagaatctt tttattgtca gtactgactc gagttattat ggacatggca tagacatata
      301 caaagcttgt tcaccatcgg aagcagtacc atcgtataaa gcagtatcca aaccacacaa
      361 agtgaaaccc attcttctat aagcatgaat agctggagca ttaacattgg taacttccaa
      421 ccacaaatga ccagcacctc tttctctggc gaattcagta gccaaaccca tcaaagctct
      481 accaacacca tgacctctat gttctggagc aacttcaata tcttcaacag tcaatcttct
      541 gttccaacca gaataagaaa caacaacgaa accagccaaa tcaccatcat caccataagc
      601 aacgaaagtt ctagaatctg gatcaccatc ttcaccagca tcggattcat catcggattc
      661 atcatctggg aaaaccttag tcaatggtgg atcaactgga acttctctca aagtgaaacc
      721 atcaccagta gcagtaactc taaaaacagt atcggtagtg aaagaaccat ccaaagcttc
      781 aatagcttca gcatcacctg gaacagaagt tctgtatcta taagcagtat catccaaagt
      841 agtagacata attgtaggat ccggttgttt atgttcggat gtgatgtgag aactgtatcc
      901 tagcaagatt ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt
      961 tatatttctc tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt
     1021 tccctgctcg caggtctgca gatgtcttac gccactaaaa tccacgccag atacgtctac
     1081 gactccagag gtaacccaac cgttgaagtt gatttcacca ccgacaaagg tttattcaga
     1141 tcaattgtcc catctggtgc ctctactggt gtccacgaag ctttggaatt gagagatggt
     1201 gacaaatcca aatggttagg taaaggtgtt ttgaaagccg ttgccaatgt taatgacatc
     1261 attgccccag ctttaataaa agccaagatc gatgttgtcg accaagctaa gattgatgaa
     1321 ttcttgttgt ccttggacgg tactccaaac aaatccaaat tgggtgccaa tgctatcttg
     1381 ggtgtttctt tggctgctgc caatgctgcc gctgctgctc aaggcattcc attgtacaaa
     1441 cacattgcca acatttccaa tgccaagaaa ggtaaattcg ttttgccagt tccattccaa
     1501 aacgttttga acggtggttc ccatgctggt ggtgctttag ctttccaaga atttatgatt
     1561 gccccaactg gtgtctccac tttctctgaa gctttgagaa ttggttcaga agtttaccac
     1621 aacttgaaat ctttgaccaa gaagaaatac ggtcaatccg ctggtaacgt cggtgacgaa
     1681 ggtggtgttg ctccagatat caaaactcca aaggaagctt tggacttgat catggatgcc
     1741 attgacaaag ccggttacaa aggtaaggtt ggtattgcca tggatgttgc tccatctgaa
     1801 ttctacaagg acggtaaata ccgggccccc cctcgagttt gaaaggtctg tcatatttct
     1861 atcaatttca aaacaaaatg ttcatcagaa aaaagccaaa aatgtctctt ctagtttctt
     1921 agtggtgtcg cataatacac aatgtcgctc aacaatccac attcccggcg catagctcaa
     1981 atcacatgac tacagctaac aattacacaa aaaaaattct ctttttgatg tagcaactat
     2041 cttcaactaa aacattttct ccttcggccc atgattgtcc tccgggtcga cagcaagccg
     2101 ttacaattga gatggaaagc gacctacctt cactcgataa ggtgcttaat tgtacttcat
     2161 ataaatctgg cccggatcta aacaaatgag ttccattaag ccgtgggttc tcaattaggg
     2221 tttttgtttt tgatttagaa aaaagagatc aagatttgtt tacaggtgat gccttttttt
     2281 agaacttatg cgttgcaaaa gttgactaac gatttctata aggtgatcca cactaattat
     2341 acaaacgtac aaacagacat acttttcctg cgttcacctg atgttggcca gatttctctc
     2401 ttcattgcat agaacataac cacactaggg caacagaaaa aaaaaaaaag tgcatcggga
     2461 agttgtgttc cattcattat atgtctacta ctgcatatga gtagcccacc caccaccacc
     2521 atagtaagtt tttgtgtatg cgcgccgtca ggttatttca tttctgaatt tttcaaccac
     2581 cttactccct ttattgttga ttgacaattt tgctcacagt aagatctttt agactccaat
     2641 taatataaaa taagtctgat tttccaattc ctgttttttc tttttttttc tgtttctatt
     2701 tctttccttt tctccctttt ttttaattct tcattcaatc atcaattgat aattcaggaa
     2761 tattacaaca aagcttgcca ccatgtctga agagttgatt aaagagaata tgcatatgaa
     2821 gttgtatatg gaaggtactg ttgataacca ccattttaaa tgtacatctg agggagaagg
     2881 taagccttac gaaggtactc aaacaatgcg tattaaagta gttgagggag gtcctttacc
     2941 attcgctttt gacatattgg caacttcatt cttatatgga tctaagacat ttattaacca
     3001 tactcaaggt ataccagatt tctttaaaca atcatttcct gaaggattca catgggagag
     3061 agtaacaact tatgaagacg gtggagtttt gactgctact caggatacat ctttgcaaga
     3121 cggttgtttg atttataatg ttaagattcg tggagtaaac ttcacttcta atggtccagt
     3181 tatgcagaag aaaacattgg gatgggaggc attcactgag acattatacc ctgctgatgg
     3241 tggattggaa ggtcgtaacg acatggcatt gaaattagta ggaggttcac acttgatagc
     3301 taatattaag actacatatc gttctaagaa accagctaaa aacttgaaaa tgcctggtgt
     3361 ttactatgtt gattatcgtt tagaaagaat taaggaagca aataacgaaa cttacgttga
     3421 gcaacatgaa gtagctgttg cacgttattg tgacttgcct tctaagttag gacacaaatt
     3481 gaactagctg cagcccggta ttgctgcatt cttgatctaa ttctaataac tgatactaag
     3541 ttttgttctt tttttgggat ttcttttttt tctaattttg attgtttttc aattttcggt
     3601 tttcaatatt attgacaaga gtcattttat tgaatatttg ttttgtttac tacattaaag
     3661 gtgataggta cttttagttt ttaaaaattg ttttgttcaa attgtttatc tttttcttct
     3721 tcttctactt gctttgtttt ctgttttcgg ttcatagttg atagctttta ataaataccc
     3781 cttttttttt acaatagtta gttctatgct tattcagtgg tttaattggt agaaaatgtg
     3841 agctccagct tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca
     3901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3961 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4021 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4081 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5341 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5401 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5461 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5521 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5581 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5641 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5701 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5761 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5821 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5881 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5941 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6001 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gacgcgccct
     6061 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6121 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6181 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6241 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6301 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6361 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6421 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6481 ttaacaaaat attaacgttt acaatttgcc attcgccatt caggctgcgc aactgttggg
     6541 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     6601 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     6661 ccagtgaatt gtaatacgac tcactatagg gcgaattg
//
