LOCUS       sequence-75201-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB63
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtctcctcc gagttgggag aggtcgatac gggtctcgta gagtccggtg atggattggt
       61 gaatgagggt ggcgtcgagg acctccttgg tggaggtgta acgcttacgg tcgatggtgg
      121 tgtcgaagta cttgaaggcg gctggggctc cgaggttggt gagggtgaag aggtggatga
      181 tgttctcggc ttgctcacgg attggcttgt cacggtgctt gttgtaggcg gagaggacct
      241 tgtcgaggtt ggcgtcggcg aggatgacac gcttggagaa ctcggagatt tgctcgatga
      301 tctcgtcgag gtagtgcttg tgttgctcga cgaagagttg cttttgctcg ttgtcctctg
      361 gggatccctt gagcttctcg tagtgggagg cgaggtagag gaagttgacg tacttggatg
      421 ggagggcgag ctcgtttccc ttttggagct ctccggcgga ggcgagcata cgcttacgtc
      481 cgttctcgag ctcgaagagg gagtactttg ggagcttgat gatgaggtcc ttcttgacct
      541 ccttgtatcc cttggcctcg aggaagtcga ttgggttctt ctcgaaggag gaacgctcca
      601 tgatggtgat tccgaggagc tccttgacgg acttgagctt cttggacttt cccttctcga
      661 ccttggcgac gacgaggacg gagtaggcga cggttgggga gtcgaatcct ccgtacttct
      721 tt
//
