LOCUS       sequence-75202-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB66
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttaccttga agaagatggt acgctcttgg acgtatccct ctggcatggc ggacttgaag
       61 aagtcgtgac gcttcatgtg gtctgggtaa cgggagaagc attggactcc gtaagtgaag
      121 gtggtgacga gggttggcca tgggactggg agctttccgg tggtgcagat gaacttgagg
      181 gtgagctttc cgtaggtggc gtctccctct ccctctcctg agacggagaa cttgtgtccg
      241 ttgacgtctc cgtcgagctc gacgaggatt gggacgactc cggtgaagag ctcctctccc
      301 ttggacatct gcaggtctcc tccgagttgg gagaggtcga tacgggtctc gtagagtccg
      361 gtgatggatt ggtgaatgag ggtggcgtcg aggacctcct tggtggaggt gtaacgctta
      421 cggtcgatgg tggtgtcgaa gtacttgaag gcggctgggg ctccgaggtt ggtgagggtg
      481 aagaggtgga tgatgttctc ggcttgctca cggattggct tgtcacggtg cttgttgtag
      541 gcggagagga ccttgtcgag gttggcgtcg gcgaggatga cacgcttgga gaactcggag
      601 atttgctcga tgatctcgtc gaggtagtgc ttgtgttgct cgacgaagag ttgcttttgc
      661 tc
//
