LOCUS       sequence-75203-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB66
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(374..392)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(413..429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(413..429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(426..448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    437..453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(461..467,468..485,486..491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    506..527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(644..661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt
       61 cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt
      121 actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa
      181 aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac
      241 tttattgtgc cgccaagaaa aaagtctcat tgcctaagag aaacatatgt ggttggaaat
      301 cttccagatc gtggatcact agtatgtggt tggaaatctt ccagatcgtg gatcagagct
      361 ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc
      421 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      481 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      541 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc c
//
