LOCUS       sequence-75207-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of Flp step-arrest mutant in yeast, URA selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Tsalik EL, Gartenberg MR
  TITLE     Curing Saccharomyces cerevisiae of the 2 micron plasmid by targeted 
            DNA damage.
  JOURNAL   Yeast. 1998 Jun 30;14(9):847-52.
  PUBMED    9818722
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-06-30"; volume: "14(9)"; pages: "847-52"
COMMENT     Sequence Label: pBIS-GALkFLP(URA)
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    38..541
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        578..682
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ataggttaat gtcatgataa taatggtttc ttagacggat cgcttgcctg taacttacac
       61 gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt tatttatttt
      121 tatgttttgt atttggattt tagaaagtaa ataaagaagg tagaagagtt acggaatgaa
      181 gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt acatatatat
      241 ttattagaca agaaaagcag attaaataga tatacattcg attaacgata agtaaaatgt
      301 aaaatcacag gattttcgtg tgtggtcttc tacacagaca agatgaaaca attcggcatt
      361 aatacctgag agcaggaaga gcaagataaa aggtagtatt tgttggcgat ccccctagag
      421 tcttttacat cttcggaaaa caaaaactat tttttcttta atttcttttt ttactttcta
      481 tttttaattt atatatttat attaaaaaat ttaaattata attattttta tagcacgtga
      541 tgaaaaggac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgt
//
