LOCUS       sequence-75217-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; 5' entry vector for 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: p5E_GGWDest-
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..84
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     89..108
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        89..107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(180..303)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(304..320)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     342..359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    476..497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(512..517,518..535,536..542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    550..566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     555..577
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     574..590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        608..626
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     608..625
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      720..729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      724..733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca
       61 gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga tatcgcggcc
      121 gccctgcagc tctagagctc gaattctaca ggtcactaat accatctaag tagttggttc
      181 atagtgactg catatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
      241 aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt ttgtacaaac
      301 ttggataccg tcgacctcga gccttaagag accccgagcg cagcgagtca gtgagcgagg
      361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      421 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      481 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      541 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      601 ccaagctatt taggtgacac tatagaatac tcaagctatg catccaacgc gttgggagct
      661 ctcccatatg gtcgacctgc aggcggccgc gaattcacta gtgatatcga attcccgcgg
      721 ccgccatggc ggccgggagc atgcgacgtc
//
