LOCUS       sequence-75218-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; 5' entry vector for 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: p5E_GGWDest+
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..88
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     93..112
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        93..111
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(184..307)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(308..324)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     346..363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    480..501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(516..521,522..539,540..546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    554..570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     559..581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     578..594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        612..630
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     612..629
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      724..733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
       61 gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcgc
      121 ggccgccctg cagctctaga gctcgaattc tacaggtcac taataccatc taagtagttg
      181 gttcatagtg actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      241 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttttgtac
      301 aaacttggat accgtcgacc tcgagcctta agagaccccg agcgcagcga gtcagtgagc
      361 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      481 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      541 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      601 tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg
      661 agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgata tcgaattccc
      721 gcggccgcca tggc
//
