LOCUS       sequence-75221-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; Few restriction sites 
            for subcloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGDestSC+ATG
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..89
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(133..149)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(134..150)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     172..189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    306..327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(342..347,348..365,366..372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    380..396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     385..407
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     404..420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        438..456
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     438..455
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      550..559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      554..563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(594..612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(619..636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(619..635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(628..650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
       61 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatatca
      121 gattaggccg gccgataccg tcgacctcga gccttaagag accccgagcg cagcgagtca
      181 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      241 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      301 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      361 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      421 catgattacg ccaagctatt taggtgacac tatagaatac tcaagctatg catccaacgc
      481 gttgggagct ctcccatatg gtcgacctgc aggcggccgc gaattcacta gtgatatcga
      541 attcccgcgg ccgccatggc ggccgggagc atgcgacgtc gggcccaatt cgccctatag
      601 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      661 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctgg
//
