LOCUS       sequence-75261-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     pBabe-IRES-EGFP plasmids
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe bleo IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..478
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 ttagagtgtg aggaggcttt tttggaggcc taggcttttg caaaaagctt gccagctggg
       61 gcgccctctg gtaaggttgg gaagccctgc agatctgatc accatggcca agttgaccag
      121 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
      181 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
      241 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
      301 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
      361 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
      421 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgacc
      481 gacgccgacc aacaccgccg gtccgacgcg gcccgacggg tccgaggcat cgataaaata
      541 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
      601 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
      661 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
      721 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
      781 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
      841 tccccagatg cggtccagcc ctcagcagtt tctagagaac catca
//
