LOCUS       sequence-75277-e        8095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-On inducible expression of Myc-tagged hCEP120.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Lin YN, Wu CT, Lin YC, Hsu WB, Tang CJ, Chang CW, Tang TK
  TITLE     CEP120 interacts with CPAP and positively regulates centriole 
            elongation.
  JOURNAL   J Cell Biol. 2013 Jul 22;202(2):211-9. doi: 10.1083/jcb.201212060. 
            Epub 2013 Jul 15.
  PUBMED    23857771
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201212060"; journalName: "J Cell Biol"; date: 
            "2013-07-22- 22"; volume: "202"; issue: "2"; pages: "211-9"
COMMENT     Sequence Label: hCEP120-Myc
FEATURES             Location/Qualifiers
     source          1..8095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4017..4046
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4062..4079
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4102..4119)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4108..4332
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4378..4806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4465..4484)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4675..4696
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4815..4835)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4820..5150
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5001..5136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5063..5082
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5198..5245
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5264..5638
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5768..5889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5805..5824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5859..5878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5938..5954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5938..5954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5951..5973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5962..5978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5986..5992,5993..6010,6011..6016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6031..6052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6169..6186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6340..6928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6420..6439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7099..7890,7891..7959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7722..7741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7960..8064)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggaacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attccaccat ggtctccaaa tccgaccaat tgctcatcgt cgtgtccatc ctagaaggtc
     1081 ggcatttccc caaacgtcca aagcatatgc ttgtagtgga agcaaagttt gatggagaac
     1141 agttggctac tgatcctgtg gaccacactg accagccaga atttgctact gagttagctt
     1201 gggaaattga caggaaagcg cttcatcagc acaggctaca gcgtactcct atcaaactcc
     1261 aatgttttgc cttggatcct gtaacttcag ccaaggaaac cataggttac atcgttctgg
     1321 atttaagaac cgctcaagaa acaaagcagg caccaaaatg gtaccagttg ctgagtaata
     1381 aatacaccaa attcaagtct gagatacaga taagtattgc tttggaaacc gatacaaagc
     1441 caccagtgga tagctttaaa gcaaaggggg ctccccctcg agatggaaaa gtacctgcca
     1501 tcctggctgg acttgacccc agggacattg tggctgtgct gaatgaagag ggaggctacc
     1561 atcagattgg accagcagaa tactgtactg actcctttat tatgtcagtg accatagcat
     1621 ttgctaccca gttggaacag ttaattccat gtaccatgaa acttccagaa agacagcctg
     1681 agtttttctt ttactactct ttactgggaa atgatgttac aaatgaaccc ttcaatgatt
     1741 taatcaaccc aaactttgag ccagagagag catcagttcg catccgtagc agtgtagaaa
     1801 ttcttcgtgt ttacctggct cttcagtcta aactgcagat tcatctctgc tgtggagacc
     1861 agtcacttgg aagtacagaa atacctttaa ctggattact taaaaagggc agtacagaaa
     1921 tcaaccagca cccagtcaca gtcgaaggtg cttttaccct tgaccctcca aacagagcca
     1981 aacagaagct tgcacctatt cctgtggagc tagccccaac tgtgggagtg tctgtggctc
     2041 tgcagagaga aggcatagac tcccagtctt taattgaatt aaagacccag aatgaacatg
     2101 agccagagca ttcaaagaag aaagttttaa cccccataaa ggagaagaca cttactgggc
     2161 caaaatcacc aacagtgtcc cctgttccat ctcacaacca gtcacctcca acaaaagatg
     2221 atgcaacaga aagtgaagtg gaaagtttac agtatgataa ggacaccaaa ccaaatccaa
     2281 aagccagttc ttctgtacct gcttcactgg cccagctagt gactacatcc aatgcttcag
     2341 aagtagcttc aggacagaag attgctgtac cagcaacatc acatcatttt tgcttttcaa
     2401 tagacttaag gagtatacat gccttggaga ttggttttcc aatcaactgt atattaaggt
     2461 actcatatcc attctttgga agtgcagctc ctattatgac taatcctcct gtagaagttc
     2521 ggaaaaacat ggaagttttt cttccccagt cttactgtgc atttgatttt gcaactatgc
     2581 ctcatcagct gcaagacacc ttcttaagga ttccattact ggttgaacta tggcacaagg
     2641 ataaaatgag taaagattta cttctgggaa ttgcgagaat ccagctttct aacatcttgt
     2701 cttcagaaaa aactcgtttt ttaggttcta atggtgaaca gtgttggcgt caaacttaca
     2761 gtgaaagtgt gcctgttata gcagcacaag gatcaaataa caggatagca gatctttctt
     2821 acacagtgac tgtagaagat tatggactag taaaaatgcg tgagattttt atctctgatt
     2881 catctcaggg tgtatctgcc gtacagcaaa agccgtcttc tcttcctcca gcaccttgtc
     2941 cttcagagat ccagacagag cctcgtgaaa cgttagaata caaagcagca cttgagctag
     3001 aaatgtggaa ggagatgcaa gaagatatat ttgaaaatca gctgaagcag aaagaactgg
     3061 ctcatatgca ggctcttgca gaggaatgga agaaaaggga ccgagaaaga gaatcactag
     3121 taaagaaaaa ggtggctgaa tatactattc tagaaggaaa acttcaaaaa actctaattg
     3181 acttggagaa gcgagagcag cagcttgcta gtgtggaatc agagcttcaa agagaaaaaa
     3241 aggaactgca atcagaacgt cagcggaacc tgcaagaact gcaggactct atccgtaggg
     3301 ccaaagagga ctgtattcac caagtagaac tagaaaggtt aaaaatcaaa cagctcgaag
     3361 aggataaaca ccgccttcag caacagctta atgatgctga aaataagtat aagattttgg
     3421 aaaaagagtt ccaacagttc aaggaccagc aaaacaacaa accagaaatc cgtctacagt
     3481 ctgaaataaa tcttctcacc ttggaaaagg ttgaacttga aagaaagttg gaatctgcaa
     3541 ctaagtctaa actgcattac aagcagcagt ggggacgagc tttgaaagaa cttgccagac
     3601 ttaaacagag ggagcaagaa agtcaaatgg ctcgtcttaa aaaacagcag gaagaattgg
     3661 aacacatgag actacgttac cttgccgctg aggaaaaaga tacagtaaaa accgagcgac
     3721 aagaattgtt ggatataaga aatgaattga acaggttaag gcaacaagag caaaaacaat
     3781 accaggattc cacagagatt gcaagtggaa aaaaggatgg cccccatggc agtgtattgg
     3841 aagaaggttt ggacgattat ttgactcgcc tgatagaaga aagggatact ttgatgagaa
     3901 cgggtgtgta taatcacgag gatcgaataa taagtgaact cgaccgacag atcagagaga
     3961 ttttggcaaa aagcaatgcc agtaatacgc ggccgctcga gtctagaggg cccttcgaac
     4021 aaaaactcat ctcagaagag gatctgaata tgcataccgg tcatcatcac catcaccatt
     4081 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4141 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4201 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4261 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     4321 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     4381 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4441 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4501 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg
     4561 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4621 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4681 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4741 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4801 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg
     4861 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5101 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5161 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     5221 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     5281 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     5341 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     5401 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     5461 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     5521 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     5581 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     5641 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5701 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5761 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5881 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     5941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     6541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6781 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7381 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
