LOCUS       sequence-75282-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for ectopic constitutive expression of two mycobacterial proteins in
            one vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pST-2K
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(166..265)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             610..627
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(657..700)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cttggtcgat accaagccat ttccgctgaa tatcggggag ctcaccgcca gaatcggtgg
       61 ttgtggtgat gtacgtggcg aactccgttg tagtgcctgt ggtggcatcc gtggcggtac
      121 cgcctgacgc cccccggatc agtactagta agggtgggcg cgccgaccca gctttcttgt
      181 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
      241 tcagtcaaaa taaaatcatt atttgccatc cagctgatac taatattgga tcgtcggcac
      301 cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca tccccacggt
      361 gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac gcacaggccc
      421 ggtgtgagaa gggtctctga cgagcgggag aactccctat cagtgataga gtttgtcctc
      481 cctatcagtg atagataggc tctgggagta cccgtgtgta cgaccagcac ggcatacatc
      541 atttcgacgc cgagagattc gccgcccgaa atgagcacga tccgcatgcg gaggaatcac
      601 ttcgcaatgc accaccacca ccaccatatg ggatccgata tctctagagc ggccgcataa
      661 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat agggccgccc ccttcaccca
      721 gctgagtact aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc
      781 gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg
      841 ttgatctgtg cgttcgcacg caca
//
