LOCUS       sequence-75295-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast expression of Cre recombinase fused to the estrogen binding 
            domain (EBD), which confers estradiol-dependent control to Cre 
            recombinase .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cheng TH, Chang CR, Joy P, Yablok S, Gartenberg MR
  TITLE     Controlling gene expression in yeast by inducible site-specific 
            recombination.
  JOURNAL   Nucleic Acids Res. 2000 Dec 15;28(24):E108.
  PUBMED    11121495
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2000-12-15"; volume: "28(24)"; pages: "E108"
COMMENT     Sequence Label: pSH62-EBD
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2..20)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    68..571
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        601..705
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagggt
       61 ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa
      121 attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat
      181 gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct
      241 caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct
      301 gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg
      361 tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt
      421 tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac
      481 aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata
      541 cgaggcgcgt gtaagttaca ggcaagcgat ccgtcaggtg gcacttttcg gggaaatgtg
      601 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      661 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      721 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      781 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
//
