LOCUS       sequence-75298-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; Few restriction sites 
            for subcloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGDestSC+ATG
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(162..180)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(163..180)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(198..214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(211..233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    222..238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(246..252,253..270,271..276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    291..312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(429..446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     468..484
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     469..485
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(506..524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(529..545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..666)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(725..744)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
       61 atggcggccg cgggaattcg atatcactag tgaattcgcg gccgcctgca ggtcgaccat
      121 atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat
      181 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      301 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      421 tccgcttcct cgctcactga ctcgctgcgc tcggggtctc ttaaggctcg aggtcgacgg
      481 tatcggccgg cctaatctga tatcccctat agtgagtcgt attacatggt catagctgtt
      541 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
      601 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
      661 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
      721 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
      781 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
      841 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
      901 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
      961 aaaacagcat tccaggtatt agaagaatat cct
//
