LOCUS       sequence-75305-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX2
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..764
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     primer_bind     complement(805..822)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgacctcggg ctcgggcgga aagtcttacc caactgtgag tgctgattac caggacgccg
       61 ttgagaaggc gaagaagaag ctcagaggct tcatcgctga gaagagatgc gctcctctaa
      121 tgctccgttt ggcattccac tctgctggaa cctttgacaa gggcacgaag accggtggac
      181 ccttcggaac catcaagcac cctgccgaac tggctcacag cgctaacaac ggtcttgaca
      241 tcgctgttag gcttttggag ccactcaagg cggagttccc tattttgagc tacgccgatt
      301 tctaccagtt ggctggcgtt gttgccgttg aggtcacggg tggacctaag gttccattcc
      361 accctggaag agaggacaag cctgagccac caccagaggg tcgcttgccc gatcccacta
      421 agggttctga ccatttgaga gatgtgtttg gcaaagctat ggggcttact gaccaagata
      481 tcgttgctct atctgggggt cacactattg gagctgcaca caaggagcgt tctggatttg
      541 agggtccctg gacctctaat cctcttattt tcgacaactc atacttcacg gagttgttga
      601 gtggtgagaa ggaaggtctc cttcagctac cttctgacaa ggctcttttg tctgaccctg
      661 tattccgccc tctcgttgac aaatatgcag cggacgaaga tgccttcttt gctgattacg
      721 ctgaggctca ccaaaagctt tccgagcttg ggtttgctga tgcctaactc gagtctagag
      781 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      841 tttgcccctc ccccgtgcct tccttgaccc tgga
//
