LOCUS       sequence-75312-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB66
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    124..145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(160..165,166..183,184..190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    198..214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     203..225
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     222..238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     222..238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        259..277
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(385..708)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(718..792)
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="MSRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
       61 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      121 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      181 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      241 tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg agctctgatc
      301 cacgatctgg aagatttcca accacatact agtgatccac gatctggaag atttccaacc
      361 acatatgttt ctcttaggca atgagacttt tttcttggcg gcacaataaa gtttcctctt
      421 tttgttgggt cactcaacga acacaataca aatatttgag cgctcgcatt tttctcaacc
      481 tttcgggttt aaagcacttc aaaatggaat tgaagagaga ttgtgaaagt acatcagaca
      541 gaatcacatt ttgatagcat tcacttcact cagatgcaaa cttttcagtg actataaaaa
      601 gtcataaaat aataacatta aaaaagcaaa taaattagcg agagaatttt ttgacaaaaa
      661 gaaagaagag tgatagagaa gaagggaatg cttgaaagga tcttgcatga attcctagtt
      721 ctcgacctcc ttggcgagct tcttggcgtt ctcggagagc ttggttgggt tggccttacg
      781 acgacgggac atctgcagct tgtagagctc gtccattccg tgggtgattc cggcagcggt
      841 gacgaactcc ttgaggacca tgtggtcacg cttctcgttt gggtccttgg agagggcgga
      901 ttgggtggag aggtagtggt tgtctgggag gaggactggt ccgtctccga ttggggtgtt
      961 ttgttggtag tggtcggcga gttggacaga tccgt
//
