LOCUS       sequence-75322-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 V5-DEST 3XFlag-SRSF3
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             137..202
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(697..721)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             747..788
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(828..847)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    872..920
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac
      121 aaaaaagcag gctatggact acaaagacca tgacggtgat tataaagatc atgatatcga
      181 ttacaaggat gacgatgaca agggccatcg tgattcctgt ccattggact gtaaggttta
      241 tgtaggcaat cttggaaaca atggcaacaa gacggaattg gaacgggctt ttggctacta
      301 tggaccactc cgaagtgtgt gggttgctag aaacccaccc ggctttgctt ttgttgaatt
      361 tgaagatccc cgagatgctg ctgatgcagt ccgagagcta gatggaagaa cactatgtgg
      421 ctgccgtgta agagtggaac tgtcgaatgg tgaaaaaaga agtagaaatc gtggcccacc
      481 tccctcttgg ggtcgtcgcc ctcgagatga ttatcgtagg aggagtcctc cacctcgtcg
      541 cagatctcca agaaggagaa gcttctctcg cagccggagc aggtcccttt ctagagatag
      601 gagaagagag agatcgctgt ctcgggagag aaatcacaag ccgtcccgat ccttctctag
      661 gtctcgtagt cgatctaggt caaatgaaag gaaatagccc agctttcttg tacaaagtgg
      721 ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
      781 attctacgcg taccggttag taatgagttt aaacggggga ggctaactga aacacggaag
      841 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      901 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
      961 taccccaccg agaccccatt ggggccaata cgcccg
//
