LOCUS       sequence-75324-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir-128-1
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(492..516)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             869..910
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(950..969)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac
      121 aaaaaagcag gctcatatgc aaagccctag ctgttttctg tgtagctttt attattctta
      181 tgactcttga caagtttgta gcttcaccat atacatttaa tattttgcaa taattggcct
      241 tgttcctgag ctgttggatt cggggccgta gcactgtctg agaggtttac atttctcaca
      301 gtgaaccggt ctctttttca gctgcttcct ggcttctttt tactcaggtt tccactgctt
      361 ttttgctttt tttaatgctg tatgaaggtg ttaacatttg tttatatttt tcattaattg
      421 taataccttt aaatcatgca tcatactcag aaatagggat tagaatttaa gtgacatctt
      481 tggccaagct tacccagctt tcttgtacaa agtggttgat ctagagggcc cgcagtgggg
      541 tcagcttcta ccggggcggc gtcccggggt cttggaactg catgcagact gcctgcttgg
      601 gaaacatact tctttatatg cccatatgga cctgctaagc tatggaatgt aaagaagtat
      661 gtatctcagg ccgggacctc tctcgccgca ctgaggggca ctccacacca cgggggccgc
      721 cgcggccgac agccagccag gcggctgtgg tgggggtgag gctggggcgg caggctgcct
      781 gggcgttggt ggacggtcgt gtccccgcca gcattgttct gtcactttgc ctgtgtcagc
      841 aggtggaagt gctcaccaga cattcgaagg taagcctatc cctaaccctc tcctcggtct
      901 cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga
      961 aggagacaat accggaagga acccgcgcta tgacggca
//
