LOCUS       sequence-75328-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of HA and FLAG-tagged murine CAD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Ben-Sahra I, Howell JJ, Asara JM, Manning BD
  TITLE     Stimulation of de novo pyrimidine synthesis by growth signaling 
            through mTOR and S6K1.
  JOURNAL   Science. 2013 Mar 15;339(6125):1323-8. doi: 10.1126/science.1228792.
            Epub 2013 Feb 21.
  PUBMED    23429703
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1228792"; journalName: "Science"; date: 
            "2013-03-15- 15"; volume: "339"; issue: "6125"; pages: "1323-8"
COMMENT     Sequence Label: pcDNA3-FLAG-HA-CAD
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..152
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             153..179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcg atatcgccgg
      121 ccgccatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc gattacgcta
      181 gcggccgctc gtctgcgtgc ttgccctgtc tcagctccga cccgatggcg gccctggtgt
      241 tggaggacgg gtcggtgctg cagggccggc cctttggggc cgctgtgtcg actgctgggg
      301 aagtggtgtt tcagaccggc atggtcggct acccagaggc cctcactgac ccttcctaca
      361 aagctcagat cctagtgcta acgtatcctc tcatcggcaa ctacggcatt ccctcagatg
      421 aagaggacga gttcggcctg agcaagtggt ttgaatcctc ggagatccac gtggcgggac
      481 ttgtagtggg agaatgctgt cccacaccca gccactggag tgccaactgc accctgcatg
      541 agtggctgca acagcgtggc atccccggcc tgcaaggagt ggacactcgg gagctgacta
      601 agaagttgcg ggagcaaggg tctcttttgg ggaagctggt ccagaaaggg acagagccgt
      661 cagccctgcc attcgtggac cccaatgcca ggcccctggc accagaggtc tctattaaga
      721 ctccacgggt attcaatgca gggggtgccc ctcggatctg tgctctggat tgtggcctca
      781 agtataatca gatccgatgt ctctgccagc ttggggctga agttaccgtg gtaccctggg
      841 accacgagtt agacagtcag aaatatgacg gtctcttcct aagtaatgga cccggtgacc
      901 ctgcctctta cccgggtgtg gtgtccacac tgagccgagt cttgtctgaa cctaaccccc
      961 gacctgtgtt tggaatctgc cttggacacc
//
