LOCUS       sequence-75364-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian SP-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-SPn-VP64
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacttccgtt gtgtgatcag tttggcgttc agcagctgcc gccaataatt tttcattttc
       61 ttgacaactt cttctgaggg gacgttatca ctcttccctc tagctttatc ggatcttgtc
      121 aacactttat tatcaataga atcatctttg agaaaagact ggggcacgat agcagccacg
      181 tcgtagtcgg agagccgatt gatgtccagt tcctgatcca cgtacatgtc cctgccgttc
      241 tgcaggtagt acaggtagag cttctcattc tgaagctggg tgttttcaac tgggtgttcc
      301 ttaaggattt gggaccccag ttcttttata ccctcttcaa tcctcttcat cctttcccta
      361 ctgttcttct gtcccttctg ggtagtttgg ttctctcggg ccatctcgat aacgatattc
      421 tcgggcttat gccttcccat tactttgacg agttcatcca cgaccttaac ggtctgcagt
      481 attccctttt tgatagctgg gctacctgca agattagcga tgtgctcgtg aagactgtcc
      541 ccctggccag aaacttgtgc tttctggatg tcctccttaa aggtgagaga gtcatcatgg
      601 atcaactgca tgaagttccg gttggcaaat ccatcggact taagaaaatc caggattgtc
      661 tttccactct gcttgtctcg gatcccattg atcagttttc ttgacagccg cccccatcct
      721 gtatatcggc gcctcttgag ctgtttcatg actttgtcgt cgaagagatg agcgtaagtt
      781 ttcaagcgtt cttcaatcat ctccctatct tcaaacaacg taagggtgag gacaatgtcc
      841 tcaagaatgt cctcgttctc ctcattgtcc aggaagtcct tgtctttaat gattttcagg
      901 agatcgtgat acgttcccag ggatgcgttg aagcgatcct ccactccgct gatttca
//
