LOCUS       sequence-75365-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian SP-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-SPn-VP64
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..185)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(210..230)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggtaggga tcgaaccctt tcatctagag ttaatcagca tgtccaggtc gaaatcatca
       61 agggcgtcac tgccgagcat gtcgaggtca aagtcatcaa gggcatccga accaagcatg
      121 tcaaggtcaa aatcatcgag ggcgtcactt cccagcatat ccagatcaaa atcgtccaat
      181 gcgtcagccc gtccggaacc gctggcctcc accttcctct tcttcttggg gtcagccctg
      241 ctgtctccac cgagctgaga gaggtcgatt cttgtttcat agagccccgt aattgactga
      301 tgaatcagtg tggcgtccag gacctccttt gtagaggtgt accgctttct gtctatggtg
      361 gtgtcgaagt acttgaaggc tgcaggcgcg cccaagttgg tcagagtaaa caagtggata
      421 atgttttctg cctgctccct gatgggctta tccctgtgct tattgtaagc agaaagcacc
      481 ttatcgaggt tagcgtcggc gaggatcact cttttggaga attcgcttat ttgctcgatg
      541 atctcatcaa ggtagtgttt gtgttgttcc acgaacagct gcttctgctc attatcttcg
      601 ggagaccctt tgagcttttc atagtggctg gccagataca agaaattaac gtatttagag
      661 ggcagtgcca gctcgttacc tttctgcagc tcgcccgcac tagcgagcat tcgtttccgg
      721 ccgttttcaa gctcaaagag agagtacttg ggaagcttaa tgatgaggtc ttttttgacc
      781 tctttatatc ctttcgcctc gagaaagtcg atggggtttt tttcgaagct tgatcgctcc
      841 atgattgtga tgcccagcag ttccttgacg cttttgagtt ttttagactt ccctttctcc
      901 actttggcca caaccagtac actgtaagcg actgtaggag aatcgaatcc gccgtatttc
      961 ttggggtccc aatcttttt
//
