LOCUS       sequence-75367-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from T to
            A at position 308 and S to A at position 473 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-T308A/S473A
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..56
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 ctagaggatc tggtaccacg cgtatcgata agcttatggg gagcagcaag agcaagccca
       61 agtctagaat gaacgacgta gccattgtga aggagggctg gctgcacaaa cgaggggaat
      121 atattaaaac ctggcggcca cgctacttcc tcctcaagaa cgatggcacc tttattggct
      181 acaaggaacg gcctcaggat gtggatcagc gagagtcccc actcaacaac ttctcagtgg
      241 cacaatgcca gctgatgaag acagagcggc caaggcccaa cacctttatc atccgctgcc
      301 tgcagtggac cacagtcatt gagcgcacct tccatgtgga aacgcctgag gagcgggaag
      361 aatgggccac cgccattcag actgtggccg atggactcaa gaggcaggaa gaagagacga
      421 tggacttccg atcaggctca cccagtgaca actcaggggc tgaagagatg gaggtgtccc
      481 tggccaagcc caagcaccgt gtgaccatga acgagtttga gtacctgaaa ctactgggca
      541 agggcacctt tgggaaagtg attctggtga aagagaaggc cacaggccgc tactatgcca
      601 tgaagatcct caagaaggag gtcatcgtcg ccaaggatga ggttgcccac acgcttactg
      661 agaaccgtgt cctgcagaac tctaggcatc ccttccttac ggccctcaag tactcattcc
      721 agacccacga ccgcctctgc tttgtcatgg agtatgccaa cgggggcgag ctcttcttcc
      781 acctgtctcg agagcgcgtg ttctccgagg accgggcccg cttctatggt gcggagattg
      841 tgtctgccct ggactacttg cactcc
//
