LOCUS       sequence-75368-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from T to
            D at position 308 and S to D at position 473 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-T308D/S473D
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..55
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 tagaggatct ggtaccacgc gtatcgataa gcttatgggg agcagcaaga gcaagcccaa
       61 gtctagaatg aacgacgtag ccattgtgaa ggagggctgg ctgcacaaac gaggggaata
      121 tattaaaacc tggcggccac gctacttcct cctcaagaac gatggcacct ttattggcta
      181 caaggaacgg cctcaggatg tggatcagcg agagtcccca ctcaacaact tctcagtggc
      241 acaatgccag ctgatgaaga cagagcggcc aaggcccaac acctttatca tccgctgcct
      301 gcagtggacc acagtcattg agcgcacctt ccatgtggaa acgcctgagg agcgggaaga
      361 atgggccacc gccattcaga ctgtggccga tggactcaag aggcaggaag aagagacgat
      421 ggacttccga tcaggctcac ccagtgacaa ctcaggggct gaagagatgg aggtgtccct
      481 ggccaagccc aagcaccgtg tgaccatgaa cgagtttgag tacctgaaac tactgggcaa
      541 gggcaccttt gggaaagtga ttctggtgaa agagaaggcc acaggccgct actatgccat
      601 gaagatcctc aagaaggagg tcatcgtcgc caaggatgag gttgcccaca cgcttactga
//
