LOCUS       sequence-75369-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian N. meningitidis Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMcas
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgcaagga ccgcgagaag gccgccgcca agttccgcga gtacttcccc aacttcgtgg
       61 gcgagcccaa gagcaaggac atcctgaagc tgcgcctgta cgagcagcag cacggcaagt
      121 gcctgtacag cggcaaggag atcaacctgg gccgcctgaa cgagaagggc tacgtggaga
      181 tcgaccacgc cctgcccttc agccgcacct gggacgacag cttcaacaac aaggtgctgg
      241 tgctgggcag cgagaaccag aacaagggca accagacccc ctacgagtac ttcaacggca
      301 aggacaacag ccgcgagtgg caggagttca aggcccgcgt ggagaccagc cgcttccccc
      361 gcagcaagaa gcagcgcatc ctgctgcaga agttcgacga ggacggcttc aaggagcgca
      421 acctgaacga cacccgctac gtgaaccgct tcctgtgcca gttcgtggcc gaccgcatgc
      481 gcctgaccgg caagggcaag aagcgcgtgt tcgccagcaa cggccagatc accaacctgc
      541 tgcgcggctt ctggggcctg cgcaaggtgc gcgccgagaa cgaccgccac cacgccctgg
      601 acgccgtggt ggtggcctgc agcaccgtgg ccatgcagca gaagatcacc cgcttcgtgc
      661 gctacaagga gatgaacgcc ttcgacggta aaaccatcga caaggagacc ggcgaggtgc
      721 tgcaccagaa gacccacttc ccccagccct gggagttctt cgcccaggag gtgatgatcc
      781 gcgtgttcgg caagcccgac ggcaagcccg agttcgagga ggccgacacc cccgagaagc
      841 tgcgcaccct gctggccgag aagctgagca gccgccctga ggccgtgcac gagtacgtga
      901 ctcctctgtt cgtgagccgc gcccccaacc gcaagatgag cggtca
//
