LOCUS       sequence-75370-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter hsa-mir17~20
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..52)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    405..429
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaatg
       61 tctggtgagc acttccacct gctgacacag gcaaagtgac agaacaatgc tggcggggac
      121 acgaccgtcc accaacgccc aggcagcctg ccgccccagc ctcaccccca ccacagccgc
      181 ctggctggct gtcggccgcg gcggcccccg tggtgtggag tgcccctcag tgcggcgaga
      241 gaggtcccgg cctgagatac atacttcttt acattccata gcttagcagg tccatatggg
      301 catataaaga agtatgtttc ccaagcaggc agtctgcatg cagttccaag accccgggac
      361 gccgccccgg tagaagctga ccccactgcg ggccctctag atcaaccact ttgtacaaga
      421 aagctgggta agcttaccga tcccaacctg tgtagaaagg ggtttgagtt tcccttactt
      481 ttctacagac ttttcactac cacagtcagt tttgcatgga tttgcacagc agaatatcac
      541 acagctggat gcaaacctgc aaaactaacc atagaacagt gttcagtaac aggacagttt
      601 gattgggcga caggccgaag ctggagttct acagctagca gtactttaag tgctcataat
      661 gcagtagata actaaacact acctgcacta taagcacttt agtgctacag aagctgtcac
      721 atcagataga ccaggcagat tctacatcga cacaataaaa gtacacaaaa ttagtaaaaa
      781 tcattcattt gaaggaaata gcaggccacc atcagttttg catagatttg cacaactaca
      841 ttcttcttgt agtgcaacta tgcaaaacta acagaggact gcaaacaaaa actattaaaa
      901 cacctatata cttgcttggc ttgaattatt gg
//
